Probiótico no desempenho produtivo, hematologia e migração de macrófagos do matrinxã, Brycon amazonicus
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Data
2010-07-23
Autores
Dias, Danielle de Carla [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O objetivo deste projeto foi avaliar a influência do probiótico composto de Bacillus subtillis no desenvolvimento produtivo, hematologia e migração de macrófagos de matrinxã, Brycon amazonicus, nas fases de desenvolvimento (reprodução, larva e engorda). O experimento foi dividido em três etapas: 1) Reprodução: dois grupos de matrizes foram alimentadas durante oito meses, constituindo o T1 (controle - sem adição de probiótico) e T2 (10 g de probiótico.kg- 1 de ração). No período reprodutivo, 10 fêmeas e 10 machos de cada tratamento foram selecionados para reprodução induzida. Para as análises hematológicas, utilizaram-se 20 peixes, que foram anestesiados e retiradas alíquotas de sangue por punção do vaso caudal. Outros 10 matrinxãs foram utilizados para a avaliação da atividade fagocítica dos macrófagos e também para as análises de lipídios. Os resultados mostraram que as matrizes que receberam a dieta suplementada com probiótico apresentaram aumento do número de ovócitos e, consequentemente, nas taxas de fertilização e eclosão das larvas e significativo aumento da atividade fagocítica dos magrófagos, indicando melhora no sistema imune dos reprodutores. Os parâmetros hematológicos sofreram alterações quando comparados com o momento zero e os valores de cortisol e glicose foram mais elevados em fêmeas. Os valores de lipídios totais em músculo e fígado foram inferiores nos peixes que receberam alimentação suplementada com probiótico; 2) Larvicultura: esta fase foi constituída de seis tratamentos, T1 - controle (sem adição de probiótico), T2 - 2,5 g de probiótico, T3 - 5,0 g de probiótico, T4 - 7,5 g de probiótico, T5 - 10,0 g de probiótico e T6 - 12,5 g de probiótico. As doses utilizadas foram adicionadas por litro de cultivo de Artemia salina, que serviram como bioencapsuladores do probiótico...
The aim of this study was evaluate the influence of the probiotic Bacillus subtilis in the growth performance, hematology and macrophage migration of matrinxã, Brycon amazonicus at different stages (breeding, larval and growth). The experiment was presented in three stages: 1) Reproduction: two groups of breeders were fed during 8 months: T1 (control – without probiotic) and T2 (10 g probiotic kg-1 ration). During the reproductive period, 20 animals (10 females and 10 males) from each treatment, were selected and induced for breeding whit crude carp pituitary extract. For hematological 3 analysis, 20 fish were used, anesthetized and aliquots of blood was withdrawn by caudal vessel punction. Another 10 fish were used to evaluate the phagocytic´s activity macrophages and for lipid’s analysis. The results showed that the breeders, who received the supplemented diet, showed a slight increase in the number of oocytes and, consequently, the fertilization and hatching rates of larvae, a significant increase in macrophage’s phagocyte activity, indicating an improvement in the immune system of the breeding. Hematological parameters unchanged when compared with the zero time and the values of cortisol and glucose were higher in females. The values of total lipids in muscle and liver were lower in fish fed with probiotic supplementation, 2) Hatchery: this phase consisted of 6 treatments, T1 - control (without probiotic), T2 - 2.5 g of probiotic T3 - 5.0 g of probiotic, T4 - 7.5 g of probiotic, T5 - 10.0 g of probiotic and T6 - 12.5 g of probiotic. The doses used were added per liter of Artemia salina culture, used as bioencapsulated probiotic, 4 h before the larvae feeding. After seven days, the larvae began to be fed on commercial feed (40% CP) and the probiotic... (Complete abstract click electronic access below)
The aim of this study was evaluate the influence of the probiotic Bacillus subtilis in the growth performance, hematology and macrophage migration of matrinxã, Brycon amazonicus at different stages (breeding, larval and growth). The experiment was presented in three stages: 1) Reproduction: two groups of breeders were fed during 8 months: T1 (control – without probiotic) and T2 (10 g probiotic kg-1 ration). During the reproductive period, 20 animals (10 females and 10 males) from each treatment, were selected and induced for breeding whit crude carp pituitary extract. For hematological 3 analysis, 20 fish were used, anesthetized and aliquots of blood was withdrawn by caudal vessel punction. Another 10 fish were used to evaluate the phagocytic´s activity macrophages and for lipid’s analysis. The results showed that the breeders, who received the supplemented diet, showed a slight increase in the number of oocytes and, consequently, the fertilization and hatching rates of larvae, a significant increase in macrophage’s phagocyte activity, indicating an improvement in the immune system of the breeding. Hematological parameters unchanged when compared with the zero time and the values of cortisol and glucose were higher in females. The values of total lipids in muscle and liver were lower in fish fed with probiotic supplementation, 2) Hatchery: this phase consisted of 6 treatments, T1 - control (without probiotic), T2 - 2.5 g of probiotic T3 - 5.0 g of probiotic, T4 - 7.5 g of probiotic, T5 - 10.0 g of probiotic and T6 - 12.5 g of probiotic. The doses used were added per liter of Artemia salina culture, used as bioencapsulated probiotic, 4 h before the larvae feeding. After seven days, the larvae began to be fed on commercial feed (40% CP) and the probiotic... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Fagocitose, Inoculação, Hematologia, Reprodução
Como citar
DIAS, Danielle de Carla. Probiótico no desempenho produtivo, hematologia e migração de macrófagos do matrinxã, Brycon amazonicus. 2010. ii, 112 f. Tese (doutorado) - Universidade Estadual Paulista, Centro de Aquicultura, 2010.