A glândula salivar cefálica e os compostos da secreção e de superfície cuticular em algumas espécies de abelhas eussociais (Hymenoptera, Apinae)
Carregando...
Arquivos
Data
2012-05-15
Autores
Poiani, Silvana Beani [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
As espécies de abelhas eussociais usam várias estratégias para a comunicação entre os membros da sociedade com a finalidade de coordenar as diferentes tarefas das quais esta sociedade depende. A comunicação através de sinais químicos é uma das mais difundidas e tem papel primordial na manutenção das colônias. As fontes dos compostos químicos usados na comunicação podem ser várias, mas boa parte dos compostos tem origem em glândulas exócrinas distribuídas pelo corpo das abelhas. O presente estudo abrange a análise da composição das glândulas salivares cefálicas (GSC) e da superfície cuticular através de cromatografia em fase gasosa e espectrometria de massas e de testes citoquímicos para detecção de compostos lipídicos e de atividade de fosfatases ácidas nas glândulas de operárias abelhas eussociais das espécies Apis mellifera, Scaptotrigona postica e Melipona quadrifasciata enquanto recém emergidas, trabalhando na área de cria e como forrageiras. As diferenças qualitativas e quantitativas obtidas na análise cromatográfica foram submetidas a análises estatísticas. O objetivo do estudo foi o de testar hipóteses de que há correlação entre a fisiologia das células glandulares e das substâncias presentes na secreção e na superfície cuticular, a fim de verificar: 1. Mudanças na composição celular relacionáveis à função secretora; 2. Relação entre a fase da vida/função das operárias e a composição da secreção glandular e dos compostos cuticulares, ou seja, que a composição da secreção pode funcionar na colônia como sinais, tanto de reconhecimento como de capacitação para determinada tarefa; 3. Se existe a possibilidade da contribuição da glândula para a formação do perfil químico cuticular nestas espécies de...
Eusocial bees use several strategies for communication among the members of the society with the purpose of coordinating the different tasks necessary to colony maintenance. The communication by chemical cues is one of the most frequent. There are several sources for the chemicals used in the communication, but most of them are produced by exocrine glands distributed over all body of the bees The present study includes analysis of the secretion compounds from cephalic salivary glands (CSG) and from cuticular surface by gas chromatography-mass spectrometry and citochemical tests for lipid detection and acid phosphatase activities in CSG of workers' of Apis mellifera, Scaptotrigona postica and Melipona quadrifasciata in different phases of life: newly emerged, working in brood area and foragers. The qualitative and quantitative differences obtained in the chemical analysis were submitted to statistical analyses. The objective of the study was to correlate the glandular cells physiology comparing the substances in their cytoplasm and those found in glandular secretion and on cuticular surface in order to test some hypothesis and verify: 1. Changes in the cellular compounds related to the secretory function; 2. The relationship between the phase/task of the workers' and the compounds in glandular secretion and on cuticular surface; 3. If exist the possibility of gland contribution to forming the cuticular chemical profile in these species of bees. Previous morphological studies of glandular cells showed the lipid nature of the CSG secretion, therefore, glandular citochemical analysis aimed to show the presence of these compounds with the purpose of accompanying their production dynamics, storage, origin and discharge, while the research of acid phosphatase activity aimed to accompany the cellular development... (Complete abstract click electronic access below)
Eusocial bees use several strategies for communication among the members of the society with the purpose of coordinating the different tasks necessary to colony maintenance. The communication by chemical cues is one of the most frequent. There are several sources for the chemicals used in the communication, but most of them are produced by exocrine glands distributed over all body of the bees The present study includes analysis of the secretion compounds from cephalic salivary glands (CSG) and from cuticular surface by gas chromatography-mass spectrometry and citochemical tests for lipid detection and acid phosphatase activities in CSG of workers' of Apis mellifera, Scaptotrigona postica and Melipona quadrifasciata in different phases of life: newly emerged, working in brood area and foragers. The qualitative and quantitative differences obtained in the chemical analysis were submitted to statistical analyses. The objective of the study was to correlate the glandular cells physiology comparing the substances in their cytoplasm and those found in glandular secretion and on cuticular surface in order to test some hypothesis and verify: 1. Changes in the cellular compounds related to the secretory function; 2. The relationship between the phase/task of the workers' and the compounds in glandular secretion and on cuticular surface; 3. If exist the possibility of gland contribution to forming the cuticular chemical profile in these species of bees. Previous morphological studies of glandular cells showed the lipid nature of the CSG secretion, therefore, glandular citochemical analysis aimed to show the presence of these compounds with the purpose of accompanying their production dynamics, storage, origin and discharge, while the research of acid phosphatase activity aimed to accompany the cellular development... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Abelha, Analise cromatografica, Espectrometria de massa, Citoquimica
Como citar
POIANI, Silvana Beani. A glândula salivar cefálica e os compostos da secreção e de superfície cuticular em algumas espécies de abelhas eussociais (Hymenoptera, Apinae). 2012. 131 f. Tese - (doutorado) - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro, 2012.