Análise da interação funcional entre as proteínas elF5A e Yptl em S. cerevisiae
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Data
2007-07-27
Autores
Frigieri, Mariana Carina [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O Poli ácido glutâmico (PAG) é um poliaminoácido sintético formado por unidades repetitivas de glutamato, que apresentam grupos carboxilas ao longo de sua cadeia principal. A preparação de eletrodos de carbono vítreo modificados com filmes de PAG foi investigada utilizando-se três diferentes procedimentos: eletropolimerização do ácido glutâmico (MONO); deposição direta de poli ácido glutâmico (PAG) e poli ácido glutâmico:glutaraldeído (PAG:GLU) seguida de secagem a temperatura ambiente. Após secagem, o eletrodo modificado foi submetido a ciclos sucessivos entre -0,8 a +2,0 V sob velocidade de varredura de 100 mV s-1. O ácido ascórbico foi usado como composto modelo para os eletrodos modificados, os quais apresentaram um efeito eletrocatalítico na oxidação do mesmo. Os eletrodos modificados foram caracterizados por microscopia de força atômica (AFM) e espectroscopia de impedância eletroquímica (EIS). O recobrimento sobre a superfície pela adição de PAG apresentou a melhor performance exibindo filmes nanoestruturados e uniformes com baixa resistência à transferência de carga. Os eletrodos modificados por filmes PAG foram usados para estudar a oxidação do flavonóide rutina. Os resultados mostraram um par de picos redox em +0,46/+0,43 V, usado para a pré-concentração da rutina. Uma curva analítica foi obtida no intervalo de 0,9 a 9,0 μmol L-1 e o método proposto foi aplicado para análise do antioxidante em formulação farmacêutica. Os eletrodos modificados por filmes PAG foram também usados para determinação de ácido cafêico. Um par de pico redox reversível em +0,42/+0,45 V foi obtido para o ácido cafêico em tampão B-R pH 3,5. O método foi aplicado para a determinação de ácido cafêico em amostra de vinho tinto, detectando nível de concentração de 34,8μg mL-1 sem tratamento prévio da amostra. Os filmes de PAG:GLU foram...
The poly glutamic acid (PAG) is a syntetic poliamonacid composed by repetitive glutamate units that shows carboxylic groups in the long of the principal chain. Films of PAG on the glassy carbon electrode was investigated by three diferents procedures: glutamic acid eletropomelimerization (MONO), direct deposition of the poly glutamic acid (PAG) and poly glutamic acid:glutaraldehyde (PAG:GLU), followed by drying at room temperature. After solvent evaporation, the modified electrode was submitted by cycles sucessives from -0.8 to +2.0 V at scan rate of 100 mV s-1. The ascorbic acid was used as a model compound in the modified electrodes, which presented an electrocatitic effect in the oxidation of the ascorbic acid. The modified electrodes were characterized by atomic force microscopy (AFM) and electrochemistry impedance spectroscopy (EIS). Coantings by poly glutamic acid onto the surface presented best performance by addition of PAG films that exhibits films nanostructured and uniforms with lower resistance to charge transfer. The modified electrode by PAG films were investigated to oxidation of rutin flavonoid. The results showed a redox pair of peaks at +0.46/+0.43 V, and linear analytical curve at 0.9 to 9.0 x 10-6 mol L-1. The method proposed was successful applied for rutin determination in pharmaceutical formulation. The modified electrode by PAG films was also used to determine cafeic acid. A reversible redox peak pair at +0.42/+0.45 V was obtained for cafeic acid at B-R buffer pH 3,5. The method was applied for cafeic acid determination in the red wine, where the concentration of the 34,8 μg mL-1 was obtained, without any pre-treatment of the sample. The PAG:GLU films onto the glass carbon electrode were also efficient to pre-concetrate and to determine amoxycilin (AMX) and doxorubicin (DXR) in human urine sample. Linear calibration graphs were obtained from 2,0 a 25,0 x 10-6 mol L-1...(Complete abstract click electronic access below)
The poly glutamic acid (PAG) is a syntetic poliamonacid composed by repetitive glutamate units that shows carboxylic groups in the long of the principal chain. Films of PAG on the glassy carbon electrode was investigated by three diferents procedures: glutamic acid eletropomelimerization (MONO), direct deposition of the poly glutamic acid (PAG) and poly glutamic acid:glutaraldehyde (PAG:GLU), followed by drying at room temperature. After solvent evaporation, the modified electrode was submitted by cycles sucessives from -0.8 to +2.0 V at scan rate of 100 mV s-1. The ascorbic acid was used as a model compound in the modified electrodes, which presented an electrocatitic effect in the oxidation of the ascorbic acid. The modified electrodes were characterized by atomic force microscopy (AFM) and electrochemistry impedance spectroscopy (EIS). Coantings by poly glutamic acid onto the surface presented best performance by addition of PAG films that exhibits films nanostructured and uniforms with lower resistance to charge transfer. The modified electrode by PAG films were investigated to oxidation of rutin flavonoid. The results showed a redox pair of peaks at +0.46/+0.43 V, and linear analytical curve at 0.9 to 9.0 x 10-6 mol L-1. The method proposed was successful applied for rutin determination in pharmaceutical formulation. The modified electrode by PAG films was also used to determine cafeic acid. A reversible redox peak pair at +0.42/+0.45 V was obtained for cafeic acid at B-R buffer pH 3,5. The method was applied for cafeic acid determination in the red wine, where the concentration of the 34,8 μg mL-1 was obtained, without any pre-treatment of the sample. The PAG:GLU films onto the glass carbon electrode were also efficient to pre-concetrate and to determine amoxycilin (AMX) and doxorubicin (DXR) in human urine sample. Linear calibration graphs were obtained from 2,0 a 25,0 x 10-6 mol L-1...(Complete abstract click electronic access below)
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Bioquímica, Biologia molecular, Proteína, Biochemistry
Como citar
FRIGIERI, Mariana Carina. Análise da interação funcional entre as proteínas elF5A e Yptl em S. cerevisiae. 2007. 124 f. Tese (doutorado) - Universidade Estadual Paulista, Instituto de Química, 2007.