Análise da ação do laser em baixa intensidade sobre o processo de reparo após reimplante dentário: estudo histomorfométrico e imunoistoquímico
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Data
2008-12-05
Autores
Saito, Célia Tomiko Matida Hamata [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O sucesso do reimplante dentário, mesmo nos dias atuais, é limitado, pois grande parte dos dentes é perdida devido à reabsorção radicular progressiva, justificando a busca de novas estratégias que possam controlar esse problema. Assim, foi objetivo deste estudo, avaliar por meio de análise histomorfométrica e imunoistoquímica, a ação do laser em baixa intensidade (LLLT) sobre o processo de reparo após reimplante dentário em ratos. Sessenta ratos (Rattus norvegicus albinus, Wistar) tiveram seus incisivos superiores direitos extraídos e foram aleatoriamente divididos em seis grupos: C0, C30 e C45 considerados controles, onde o reimplante dentário foi realizado imediatamente, trinta e quarenta e cinco minutos após a exodontia respectivamente, sem nenhum tratamento do ligamento periodontal remanescente e L0, L30 e L45, onde o reimplante foi realizado nos mesmos tempos extra-alveolares dos controles, porém a superfície radicular e alvéolo, com remanescentes do ligamento periodontal, foram tratados com LLLT (Arseneto de Gálio e Alumínio) antes do reimplante. Os animais foram eutanasiados após 60 dias. Os resultados evidenciaram reabsorções radiculares externas por substituição e inflamatória em todos os grupos, não apresentando diferença significativa entre o grupo controle e tratado em cada período de tempo extra-alveolar (P>0,05). A anquilose, porém, foi maior no grupo L30 em comparação com o C30 (P<0,05). Na análise imunoistoquímica houve predominância da proteína RANKL sobre a RANK e OPG no reimplante imediato (p<0,05). No período extra-alveolar de 45 minutos houve predominância da RANK sobre a RANKL (p<0,05). A proteína TRAP foi predominante nos grupos tratados com LLLT no reimplante imediato e com 30 minutos de tempo extra-alveolar (p<0,05). Esses resultados levaram a concluir que o tratamento da superfície radicular e alvéolo com LLLT, nas...
The success of the replanted tooth, nowadays, still tends to be limited because great amount of teeth are lost because of progressive root resorption, justifying the search of new strategies that can control this problem. This study investigates by histomorphometric and immunohistochemical analysis the of low-level laser terapy (LLLT) influence on in the control of root resorption on the healing process after tooth replantation. Sixty Wistar rats were submitted to extraction of their upper right incisor and randomly were allocated to one of six experimental groups. The groups: C0, C30 and C45 had been considered controls, where the teeth were replanted immediately, thirty and forty five minutes after the tooth extraction respectively, without any treatment of the remaining periodontal ligament. In the groups L0, L30 and L45, the teeth were replanted immediately, thirty and forty five minutes after tooth extraction respectively; however the root surface and dental sockets with remainders of the periodontal ligament had been treated with LLLT (gallium-aluminium-arsinide). The animals were euthanasied after 60 days. The comparative analysis did not reveal statistically significant differences in external root resorption (P>0.05) between the treatment modalities when used for tooth replantation in each extra-alveolar period, but anchylosis was bigger in L30 (P<0.05) comparing to C30. Immunohistochemical analysis showed predominant expression of RANKL on RANK and OPG in the immediate replant (p<0.05). At 45 minutes extra-alveolar time, there was predominant expression of RANKL on RANK (p<0.05). TRAP protein showed predominant expression in the LLLT groups in immediate replant and 30 minutes extra-alveolar time (P<0.05). These results suggest that the treatment of root surface and dental sockets with LLLT, in this study specification, were not able to prevent root resorption in tooth... (Complete abstract click electronic access below)
The success of the replanted tooth, nowadays, still tends to be limited because great amount of teeth are lost because of progressive root resorption, justifying the search of new strategies that can control this problem. This study investigates by histomorphometric and immunohistochemical analysis the of low-level laser terapy (LLLT) influence on in the control of root resorption on the healing process after tooth replantation. Sixty Wistar rats were submitted to extraction of their upper right incisor and randomly were allocated to one of six experimental groups. The groups: C0, C30 and C45 had been considered controls, where the teeth were replanted immediately, thirty and forty five minutes after the tooth extraction respectively, without any treatment of the remaining periodontal ligament. In the groups L0, L30 and L45, the teeth were replanted immediately, thirty and forty five minutes after tooth extraction respectively; however the root surface and dental sockets with remainders of the periodontal ligament had been treated with LLLT (gallium-aluminium-arsinide). The animals were euthanasied after 60 days. The comparative analysis did not reveal statistically significant differences in external root resorption (P>0.05) between the treatment modalities when used for tooth replantation in each extra-alveolar period, but anchylosis was bigger in L30 (P<0.05) comparing to C30. Immunohistochemical analysis showed predominant expression of RANKL on RANK and OPG in the immediate replant (p<0.05). At 45 minutes extra-alveolar time, there was predominant expression of RANKL on RANK (p<0.05). TRAP protein showed predominant expression in the LLLT groups in immediate replant and 30 minutes extra-alveolar time (P<0.05). These results suggest that the treatment of root surface and dental sockets with LLLT, in this study specification, were not able to prevent root resorption in tooth... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Cicatrização de feridas, Imuno-histoquímica, Avulsão dentária, Reimplante dentário, Terapia a laser de baixa intensidade, Imunoistoquímica, Terapia a laser, Tooth avulsion, Tooth replantation, Wound healing, Laser Therapy, Low-Level, Immunohistochemistry
Como citar
SAITO, Célia Tomiko Matida Hamata. Análise da ação do laser em baixa intensidade sobre o processo de reparo após reimplante dentário: estudo histomorfométrico e imunoistoquímico. 2008. 100 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araçatuba, 2008.