Microbiota intestinal de crianças com anomalias craniofaciais atendidas em um hospital especializado
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Data
2009-02-12
Autores
Vieira, Narciso Almeida [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O ecossistema gastrintestinal é caracterizado por interações recíprocas e dinâmicas entre o epitélio gastrintestinal, células do sistema imunológico e sua microbiota intestinal (MI), a qual desempenha atividades metabólicas importantes locais (mucosa intestinal) e sistêmicas. Avaliar a MI de crianças com malformação craniofacial atendidas em um hospital especializado e verificar a influência da antibioticoprofilaxia com cefazolina em palatoplastia foi o objetivo desse trabalho. Foram isoladas e quantificadas as bactérias anaeróbias intestinais dos gêneros Bacteroides sp, Bifidobacterium sp e Lactobacillus sp nas fezes de 11 crianças sem fissura de palato (C) e de 50, com fissura de palato (FP), no período compreendido entre maio de 2007 a setembro de 2008. Foi avaliada a MI de 18 crianças com fissura de palato após 24 horas de tratamento, em dose única na indução anestésica, com cefazolina para palatoplastia. Observou-se que a frequência de Bacteroides sp foi maior no grupo FP (p < 0,001). Para Lactobacillus sp, houve tendência a maior quantidade (p = 0,086) no grupo FP. Não houve diferença na frequência de Bifidobacterium sp entre os dois grupos de crianças (p = 0,495). Houve diminuição do número de UFCs de Bacteróides sp (p = 0,031) e Lactobacillus sp (p = 0,004) em crianças com fissura tratadas com cefazolina em cirurgia de palato. Para o gênero Bifidobacterium sp, houve tendência à diminuição nas UFCs (p = 0,063). A dificuldade de ingestão de nutrientes, aleitamento materno insuficiente, otites recorrentes, entre outras ocorrem com frequência em indivíduos com malformação craniofacial, o que pode ter influenciado na composição da MI. A associação da palatoplastia e o uso de antibiótico...
The gastrointestinal ecossystem is characterized by reciprocal and dynamic interactions between the host and the intestinal microbiota (IM), which performs local important metabolic (intestinal mucosa) and systemic activities. The purposes of this study are the assessment of the IM in children with craniofacial malformation attended at a specialized hospital and the verification of the influence of the antibiotic prophylaxis with cefazolin on palatoplasty. The intestinal anaerobic bacteria Bacteroides sp, Bifidobacterium sp and Lactobacillus sp were isolated and quantified in the feces of 11 non-cleft children (NC) and 50 cleft children (CP), from May 2007 to September 2008. After a 24-hour treatment, the IM in 18 cleft children was evaluated – in a single dose – by means of anesthetic induction with cefazolin, in palatoplasty. It was observed that the Bacteroides sp occurred more often in the CP group (p < 0.001). Regarding the Lactobacillus sp, the largest quantity (p = 0.086) tended towards the CP group. There was no frequency difference for the Bifidobacterium sp between the two groups of children (p = 0.495). There was a reduction in the number of Colony-Forming Units (CFUs) for the Bacteroides sp (p = 0.031) and the Lactobacillus sp (p = 0.004) in cleft children treated with cefazolin, in palate surgeries. Regarding the Bifidobacterium sp, there was a reduction tendency in the CFUs (p = 0.063). The difficulty to ingest nutrients, the insufficient breastfeeding, the recurrent otitis – among other things – are frequent in individuals with craniofacial malformation, which may have influenced the IM composition. The association between palatoplasty and the use of antibiotics has intensified the IM changes. New investigations must be performed in order to verify which factors related to the craniofacial malformation, the surgical treatment and the use of antimicrobians would... (Complete abstract click electronic access below)
The gastrointestinal ecossystem is characterized by reciprocal and dynamic interactions between the host and the intestinal microbiota (IM), which performs local important metabolic (intestinal mucosa) and systemic activities. The purposes of this study are the assessment of the IM in children with craniofacial malformation attended at a specialized hospital and the verification of the influence of the antibiotic prophylaxis with cefazolin on palatoplasty. The intestinal anaerobic bacteria Bacteroides sp, Bifidobacterium sp and Lactobacillus sp were isolated and quantified in the feces of 11 non-cleft children (NC) and 50 cleft children (CP), from May 2007 to September 2008. After a 24-hour treatment, the IM in 18 cleft children was evaluated – in a single dose – by means of anesthetic induction with cefazolin, in palatoplasty. It was observed that the Bacteroides sp occurred more often in the CP group (p < 0.001). Regarding the Lactobacillus sp, the largest quantity (p = 0.086) tended towards the CP group. There was no frequency difference for the Bifidobacterium sp between the two groups of children (p = 0.495). There was a reduction in the number of Colony-Forming Units (CFUs) for the Bacteroides sp (p = 0.031) and the Lactobacillus sp (p = 0.004) in cleft children treated with cefazolin, in palate surgeries. Regarding the Bifidobacterium sp, there was a reduction tendency in the CFUs (p = 0.063). The difficulty to ingest nutrients, the insufficient breastfeeding, the recurrent otitis – among other things – are frequent in individuals with craniofacial malformation, which may have influenced the IM composition. The association between palatoplasty and the use of antibiotics has intensified the IM changes. New investigations must be performed in order to verify which factors related to the craniofacial malformation, the surgical treatment and the use of antimicrobians would... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Microbiologia intestinal, Intestinos - Doenças, Intestinal microbiology
Como citar
VIEIRA, Narciso Almeida. Microbiota intestinal de crianças com anomalias craniofaciais atendidas em um hospital especializado. 2009. 85 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Medicina de Botucatu, 2009.