Expressão e função de membrso das subfamílias FGF-8 e FGF-9 em folículos antrais bovinos
Carregando...
Data
2012-02-17
Autores
Machado, Mariana Fernandes [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
Fatores de crescimento fibroblástico (FGFs) regulam o desenvolvimento folicular e a maturação oocitária através de ligação aos seus receptores (FGFRs). Os RNAm que codificam o FGFR2c e o FGFR3c foram detectados em folículos antrais bovinos. Com o objetivo de identificar FGFs que poderiam regular a foliculogênese por meios desses, investigou-se os padrões de expressão do FGF16 e FGF20 em folículos antrais bovinos, bem como os níveis de RNAm desses FGFs e seus receptores (FGFR2c e FGFR3c) durante a maturação in vitro de complexos cumulus-oócito (COCs). Além disso, dados preliminares do nosso laboratório indicativos de que a expressão do FGF17 em oócitos é regulada ao longo da maturação oocitária motivaram a investigação da função dessa proteína na maturação de COCs in vitro. Sendo assim, avaliou-se o efeito do FGF17 na expansão do cumulus e no controle da transcrição de fatores EGF-like [ampiregulina (AREG), epiregulina (EREG) e betacelulina (BTC)] e outros genes reguladores da expansão [cicloxigenase 2 (COX2), hialurona sintase 2 (HAS 2), pentraxina 3 (PTX3), proteína indutora do fator de necrose tumoral 6 (TSG6)]. Os RNAm dos FGF16 e FGF20 e as respectivas proteínas foram detectados no ovário bovino. A abundância de RNAm de FGF16 e FGF20 foi maior em células da granulosa e da teca de folículos atrésicos comparados a folículos saudáveis ou transicionais. O tratamento com FSH diminuiu a abundância de RNAm para FGF16 e FGF20 e o IGF1 inibiu FGF16 em células da granulosa cultivadas in vitro. Ao longo da maturação a expressão do FGF16 e do FGF20 foi estável no oócito, enquanto que a expressão dos receptores FGFR2c e FGFR3c foi estimulada nas células do cumulus pelo FSH. A proteína FGF16 foi localizada no oócito, células do cumulus e da teca e o FGF20 em oócitos, células do cumulus, da granulosa e da teca. O FGF17 aumentou a proporção...
Fibroblast growth factors (FGFs) regulate follicular development and oocyte maturation. Messenger RNA encoding receptors FGFR3c and FGFR2c have been detected in bovine antral follicles. Aiming to identify FGFs that can potentially regulate folliculogenesis through these receptors, the expression patterns of FGF16 and FGF20 were assessed in bovine antral follicles. Messenger RNA expression of these FGFs and their receptors (FGFR2c and FGFR3c) was also assessed in oocytes and cumulus cells, respectively, during in vitro maturation. In addition, previous data suggesting that FGF17 mRNA expression is regulated in the oocyte led us to investigate the effects of FGF17 on cumulus expansion and on the expression of EGF-like factors [amphiregulin (AREG), epiregulin (EREG) and betacelullin (BTC)] and other genes that regulate expansion [cyclooxygenase 2 (COX2), hyaluronan synthase 2 (HAS 2), pentraxin 3 (PTX3), protein-inducing tumor necrosis factor 6 (TSG6)]. FGF16 and FGF20 mRNA and proteins were detected in the bovine ovary. Messenger RNA abundance of FGF16 and FGF20 was higher in granulosa and theca cells from atretic follicles compared with healthy or transitional follicles. Treatment with FSH decreased mRNA expression of FGF16 and FGF20 and IGF1 inhibited FGF16 mRNA abundance in cultured granulosa cells. During maturation, mRNA expression of FGF16 and FGF20 was stable in the oocyte, while FGFR2c and FGFR3c were stimulated in cumulus cells by FSH. FGF16 protein was localized to the oocyte, cumulus and theca cells, and FGF20 was detected in the oocyte, cumulus, granulosa and theca cells. Supplementation of maturation medium with FGF17 increased the proportion of fully expanded COCs, but did not alter mRNA expression of COX2, HAS2, PTX3, TSG6, AREG, EREG and BTC in cumulus cells during in vitro maturation. In conclusion, FGF17 enhances bovine cumulus expansion... (Complete abstract click electronic access below)
Fibroblast growth factors (FGFs) regulate follicular development and oocyte maturation. Messenger RNA encoding receptors FGFR3c and FGFR2c have been detected in bovine antral follicles. Aiming to identify FGFs that can potentially regulate folliculogenesis through these receptors, the expression patterns of FGF16 and FGF20 were assessed in bovine antral follicles. Messenger RNA expression of these FGFs and their receptors (FGFR2c and FGFR3c) was also assessed in oocytes and cumulus cells, respectively, during in vitro maturation. In addition, previous data suggesting that FGF17 mRNA expression is regulated in the oocyte led us to investigate the effects of FGF17 on cumulus expansion and on the expression of EGF-like factors [amphiregulin (AREG), epiregulin (EREG) and betacelullin (BTC)] and other genes that regulate expansion [cyclooxygenase 2 (COX2), hyaluronan synthase 2 (HAS 2), pentraxin 3 (PTX3), protein-inducing tumor necrosis factor 6 (TSG6)]. FGF16 and FGF20 mRNA and proteins were detected in the bovine ovary. Messenger RNA abundance of FGF16 and FGF20 was higher in granulosa and theca cells from atretic follicles compared with healthy or transitional follicles. Treatment with FSH decreased mRNA expression of FGF16 and FGF20 and IGF1 inhibited FGF16 mRNA abundance in cultured granulosa cells. During maturation, mRNA expression of FGF16 and FGF20 was stable in the oocyte, while FGFR2c and FGFR3c were stimulated in cumulus cells by FSH. FGF16 protein was localized to the oocyte, cumulus and theca cells, and FGF20 was detected in the oocyte, cumulus, granulosa and theca cells. Supplementation of maturation medium with FGF17 increased the proportion of fully expanded COCs, but did not alter mRNA expression of COX2, HAS2, PTX3, TSG6, AREG, EREG and BTC in cumulus cells during in vitro maturation. In conclusion, FGF17 enhances bovine cumulus expansion... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Bovino - Reprodução, Ovarios, Celulas - Cultura e meios de cultura, Cattle - Reproduction
Como citar
MACHADO, Mariana Fernandes. Expressão e função de membrso das subfamílias FGF-8 e FGF-9 em folículos antrais bovinos. 2012. 88 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Instituto de Biociências de Botucatu, 2012.