Caracterização de uma região relacionada à característica Lignotúber em eucalipto
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Data
2011-12-20
Autores
Bortoloto, Tânia Mara [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O grupo CAGEN em parceria com a empresa Suzano de Papel e Celulose vem desenvolvendo projetos de identificação de características relacionadas ao estresse abiótico em eucalipto. A presença de lignotuber é uma característica que confere a essas espécies maior tolerância a níveis de estresse. Em 2006 foi realizado um experimento que demonstrou uma correlação entre o aumento dos níveis de estresse e o aumento da formação de lignotuber em eucalipto, até atingir a proporção de 3:1, indicando a ação de um gene com efeito principal na formação do lignotuber. Através da técnica de BSA (Bulked Segregant Analyses) em conjunto com marcadores RAPD (Random Amplified Polymorphic DNA) foi possível detectar um marcador molecular que permitiu a identificação de indivíduos não portadores do lignotuber em eucalipto. Esse marcador foi convertido em SCAR e passou a ser chamado de ELig (Eucalyptus – Lignotuber). Diante do exposto, esse trabalho teve como objetivo a caracterização dessa região genômica relacionada à presença de lignotuber em eucalipto. O marcador ELig foi eficiente em identificar indivíduos de espécies de eucalipto sem lignotuber, podendo ser incorporado a programas de seleção assistida por marcadores moleculares. As espécies Eucalyptus grandis e E. urophylla apresentaram uma única cópia da sequência ELig em seus genomas. Essa sequência mostrou identidade a um contig de EST de eucalipto de 1918 nucleotídeos do banco de dados FORESTs-FAPESP, que apresenta o domínio de proteínas DnaJ, uma grande família de proteínas com membros relacionados à resposta da planta contra estresse abiótico, incluindo proteínas heat shock e chaperonas. A identidade do ELig ao contig restringe-se...
CAGEN group in association with Suzano Pulp and Paper has developed projects to identify characteristics related to abiotic stress in Eucalyptus. Lignotuber presence is a resource which gives to these species higher levels of stress tolerance. An experiment was performed in 2006 which showed a correlation between increased stress levels and the increment of lignotuber formation, until 3:1 proportion, indicating the gene action with major effect on the lignotuber formation. Using BSA (Bulked Segregant Analyses) technique with RAPD markers (Random Amplified Polymorphic DNA) was detected a molecular marker that allowed the identification of individuals without lignotuber in Eucalyptus. This marker was converted into a SCAR (Sequenced Characterized Amplified Region) marker and was named Elig (Eucalyptus - Lignotuber). The purpose of this study is characterize this genomic region related to the lignotuber presence in Eucalyptus. The Elig marker was effective in identifying Eucalyptus without lignotuber, and it can be incorporated into marker-assisted selection programs. Eucalyptus grandis and E. urophylla presented a single copy sequence ELig in their genomes. This sequence showed identity with a eucalypt EST contig with1918 nucleotides on the FORESTs-FAPESP database, the contig displays the protein domain DnaJ, a large proteins family with members related to plant response against abiotic stress, this domain includes heat shock proteins and chaperones. The ELig identity with the contig was restricted in the 5 'ELig region, in which was not possible to identify the polymorphism in the primers pairing site, indicating that this polymorphism is found in the 3' ELig region sequence. Through the RACE 3' (Rapid Amplification of cDNA Ends)... (Complete abstract click electronic access below)
CAGEN group in association with Suzano Pulp and Paper has developed projects to identify characteristics related to abiotic stress in Eucalyptus. Lignotuber presence is a resource which gives to these species higher levels of stress tolerance. An experiment was performed in 2006 which showed a correlation between increased stress levels and the increment of lignotuber formation, until 3:1 proportion, indicating the gene action with major effect on the lignotuber formation. Using BSA (Bulked Segregant Analyses) technique with RAPD markers (Random Amplified Polymorphic DNA) was detected a molecular marker that allowed the identification of individuals without lignotuber in Eucalyptus. This marker was converted into a SCAR (Sequenced Characterized Amplified Region) marker and was named Elig (Eucalyptus - Lignotuber). The purpose of this study is characterize this genomic region related to the lignotuber presence in Eucalyptus. The Elig marker was effective in identifying Eucalyptus without lignotuber, and it can be incorporated into marker-assisted selection programs. Eucalyptus grandis and E. urophylla presented a single copy sequence ELig in their genomes. This sequence showed identity with a eucalypt EST contig with1918 nucleotides on the FORESTs-FAPESP database, the contig displays the protein domain DnaJ, a large proteins family with members related to plant response against abiotic stress, this domain includes heat shock proteins and chaperones. The ELig identity with the contig was restricted in the 5 'ELig region, in which was not possible to identify the polymorphism in the primers pairing site, indicating that this polymorphism is found in the 3' ELig region sequence. Through the RACE 3' (Rapid Amplification of cDNA Ends)... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Melhoramento genetico, Eucalipto - Melhoramento genético, Eucalyptus, Breeding
Como citar
BORTOLOTO, Tânia Mara. Caracterização de uma região relacionada à característica Lignotúber em eucalipto. 2011. 80 f. Tese (doutorado) - Universidade Estadual Paulista, Instituto de Biociências de Botucatu, 2011.