Avaliação do potencial antimicrobiano de Pouteria spp. e de triterpenos quinonametídeos com enfoque no Helicobacter pylori
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Data
2012-12-13
Autores
Nogueira, Leonardo Gorla [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
A resistência aos antibióticos é reconhecida pela Organização Mundial de Saúde (OMS) como a maior ameaça para o tratamento de doenças infecciosas. Uma estratégia importante para ajudar a combater o problema da resistência envolve a descoberta e desenvolvimento de novos agentes capazes de suprimir parcial ou totalmente os mecanismos de resistência microbiana. As plantas de cerrado são conhecidas como uma fonte de compostos de interesse biotecnológico elevado com aplicações na indústria farmacêutica e de alimentos. Dessa maneira, tornou-se relevante avaliar a atividade antimicrobiana dos extratos de Pouteria torta e Pouteria ramiflora (Sapotaceae), da fração enriquecida de Salacia campestris contendo netzaucoieno e maitenina (FrMN) e das substâncias isoladas netzaucoieno de Salacia campestris (Hippocrateaceae) e maitenina de Maytenus ilicifolia (Celastraceae), pela técnica de diluição em microplacas frente aos micro-organismos Staphylococcus aureus ATCC 25923, Salmonela sp. ATCC 19196, Bacillus subtilis ATCC 19659, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus multi-resistente isolado de paciente hospitalizado, Helicobacter pylori ATCC 43504 e Candida albicans ATCC 18804. Além disso, também foi utilizada a técnica de Checkerboard frente ao H. pylori e Time Kill frente aos micro-organismos que apresentaram sensibilidade às substâncias testadas. Os resultados obtidos demostraram que o extrato de P. torta inibiu o crescimento das cepas de S. aureus, B. subtilis e E. coli, enquanto as cepas de S. aureus, B. subtilis, E. coli, P. aeruginosa, S. setubal e C. albicans foram mais sensíveis frente ao extrato de P. ramiflora. Das amostras avaliadas, a maitenina e a FrMN foram as que apresentaram as atividades mais promissoras contra bactérias...
The antibiotic resistance is known by the World Health Organization (WHO) as the biggest threat to the treatment of infectious diseases. An important strategy to help tackle resistance involves the discovery and development of new agents able to partially or totally suppress the microbial resistance mechanisms. “Cerrado” plants are known as a source of compounds of high biotechnological interest with applications in the pharmaceutical industry and the food industry. In this manner, it has become relevant to evaluate the antimicrobial activity of the extracts of the Pouteria torta and Pouteria ramiflora (Sapotaceae), of the enriched fraction of Salacia campestris containing netzaucoien and maytenin (FrMN) and of the isolated substances netzaucoyen of Salacia campestris (Hippocrateaceae) and maytenin of Maytenus ilicifolia (Celastraceae), through the technique of dilution in microplates against microorganisms Staphylococcus aureus ATCC 25923, Salmonela sp. ATCC 19196, Bacillus subtilis ATCC 19659, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus multi-resistant isolated of a hospitalized patient, Helicobacter pylori ATCC 43504 and Candida albicans ATCC 18804. Other than that, the Checkerboard technique against the H. pylori and Time Kill against the microorganisms that were sensitive to the tested substances. The obtained results demonstrated that the P. torta extract inhibited the S. aureus, B. subtilis and E. coli growth, while the P. ramiflora extract, the strain S. aureus, B. subtilis, E. coli, P. aeruginosa, S. setubal and C. albicans were more sentitive. Of the evaluated compounds, the maytenin and the FrMN were within those who presented the most prominent activities against the bacteria Gram-positive S aureus (ATCC) and S. aureus multi-resistente, and B. subtilis... (Complete abstract click electronic access below)
The antibiotic resistance is known by the World Health Organization (WHO) as the biggest threat to the treatment of infectious diseases. An important strategy to help tackle resistance involves the discovery and development of new agents able to partially or totally suppress the microbial resistance mechanisms. “Cerrado” plants are known as a source of compounds of high biotechnological interest with applications in the pharmaceutical industry and the food industry. In this manner, it has become relevant to evaluate the antimicrobial activity of the extracts of the Pouteria torta and Pouteria ramiflora (Sapotaceae), of the enriched fraction of Salacia campestris containing netzaucoien and maytenin (FrMN) and of the isolated substances netzaucoyen of Salacia campestris (Hippocrateaceae) and maytenin of Maytenus ilicifolia (Celastraceae), through the technique of dilution in microplates against microorganisms Staphylococcus aureus ATCC 25923, Salmonela sp. ATCC 19196, Bacillus subtilis ATCC 19659, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus multi-resistant isolated of a hospitalized patient, Helicobacter pylori ATCC 43504 and Candida albicans ATCC 18804. Other than that, the Checkerboard technique against the H. pylori and Time Kill against the microorganisms that were sensitive to the tested substances. The obtained results demonstrated that the P. torta extract inhibited the S. aureus, B. subtilis and E. coli growth, while the P. ramiflora extract, the strain S. aureus, B. subtilis, E. coli, P. aeruginosa, S. setubal and C. albicans were more sentitive. Of the evaluated compounds, the maytenin and the FrMN were within those who presented the most prominent activities against the bacteria Gram-positive S aureus (ATCC) and S. aureus multi-resistente, and B. subtilis... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Helicobacter pylori, Agentes antiinfecciosos, Sapotacea, Pouteria, Produtos naturais, Plantas dos cerrados, Microbiologia
Como citar
NOGUEIRA, Leonardo Gorla. Avaliação do potencial antimicrobiano de Pouteria spp. e de triterpenos quinonametídeos com enfoque no Helicobacter pylori. 2012. 107 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, 2012.