Potencial estrogênico, antimutagênico e antigenotóxico de extratos e substâncias isoladas do gênero Syngonanthus (Eriocaulaceae) e efeito inibidor na expressão de genes por xantonas de Syngonanthus nitens
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2013-09-05
Autores
Oliveira-Höhne, Ana Paula [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Os extratos metanólicos dos capítulos de quatro espécies, pertencentes à família Eriocaulaceae, Syngonanthus dealbatus (Sd), S. macrolepsis (Sm), S. nitens (Sn) e S. suberosus (Ss), suas substâncias isoladas, 1 (luteolina), 2 (1,5,7-trihidroxi-3,6-dimetoxixantona), 3 (1,3,6,8-tetrahidroxi-2,5-dimetoxixantona), 4 (1,3,6,8-tetrahidroxi-5-metoxixantona), 5 (7-metoxiluteolina-8-c--glicopiranosídeo), 6 (7-metoxiluteolina-6-c--glicopiranosídeo), 7 (7,3’-dimetoxiluteolina-6-c--glicopiranosídeo), 8 (6-hidroxiluteolina) e a mistura M (A-1,3,6-trihidroxi-2-metoxixantona e B-1,3,6-triidroxi-2,5-dimetoxixantona) foram avaliados quanto ao seu potencial em causar mutações e também de prevenir o acontecimento dessas, através do teste de Ames e verificou-se que todos os extratos e suas substâncias isoladas não foram considerados mutagênicos sob as condições testadas, e que os mesmos apresentaram um efeito antimutagênico de forte a moderado contra a aflatoxina B1 (AFB1), o benzo[a]pireno (BaP), o 4-nitro-o-fenilenodiamino (NPD), a mitomicina C (MMC) e a azida sódica (AS); quanto ao potencial em causar danos no DNA e de prevenir e/ou reparar os danos causados por agentes genotóxicos por meio do ensaio Cometa, que identificou os extratos Sd, Sn e Ss e as flavonas 1 e 5 como causadores de dano significativo no DNA das células HepG2, após 4 horas de tratamento, dano esse que foi reparado pelas células, visto que nenhum efeito genotóxico foi observado após 24 horas. E ainda Sm, Sn e Ss e todas as isoladas, exceto 8, foram capazes de inibir os danos causados pelo agente genotóxico MMS no DNA; quanto ao seu potencial citotóxico frente às células cancerosas HepG2 e MCF-7/BUS, que identificou que todos os extratos, exceto Sm, e todas as substâncias isoladas foram capazes de induzir morte das células HepG2 significativamente, com a maioria das amostras alcançando...
The methanol extracts of capitula of four species belonging to the Eriocaulaceae family, Syngonanthus dealbatus (Sd), S. macrolepsis (Sm), S. nitens (Sn) and S. suberosus (Ss), its isolated compounds 1 (luteolin), 2 (1,5,7-trihydroxy-3,6-dimethoxyxanthone), 3 (1,3,6,8-tetrahydroxy-2,5-dimethoxyxanthone), 4 (1,3,6,8-tetrahydroxy-5-methoxyxanthone), 5 (7-methoxyluteolin-8-C--glucopyranoside), 6 (7-methoxyluteolin-6-C--glucopyranoside), 7 (7,3'-dimethoxyluteolin-6-C--glucopyranoside), 8 (6-hydroxyluteolin) and the mixture M (A-1,3,6-trihydroxy-2-methoxyxanthone and B-1,3,6-trihydroxy-2,5-dimethoxyxanthone) were evaluated for their potential to cause mutations and also to prevent the occurrence of them, using the Ames test, and it was found that all extracts and their isolated compounds were not considered mutagenic under the test conditions, and they also showed a strong to moderate antimutagenic effect against aflatoxin B1 (AFB1), benzo[a]pyrene (BaP), 4-nitro-o-phenilenodiamine (NPD), mitomycin C (MMC) and sodium azide (SA). They were also evaluated for the potential to cause DNA damage and to prevent and/or repair the damage caused by genotoxic agents, using the Comet assay, which identified the extracts Sd, Sn, Ss and the flavones 1 and 5 as causing significant DNA damage, after 4 hours of treatment. Such damage has been repaired by the cells, since no genotoxic effect was observed after 24 hours. Also Sm, Sn, Ss and all isolated compounds, except 8, were able to inhibit the damage caused by the genotoxic agent MMS. They were evaluated for their potential to cause cancer cells (HepG2 and MCF-7/BUS) death, and we found that all extracts, except Sm, and all the compounds were able to induce significant death of HepG2 cells, with most of the samples reaching 50% cell death. In MCF-7/BUS cells, after 24 hours of treatment, values were lower than 50% cell death... (Complete abstract click electronic access below)
The methanol extracts of capitula of four species belonging to the Eriocaulaceae family, Syngonanthus dealbatus (Sd), S. macrolepsis (Sm), S. nitens (Sn) and S. suberosus (Ss), its isolated compounds 1 (luteolin), 2 (1,5,7-trihydroxy-3,6-dimethoxyxanthone), 3 (1,3,6,8-tetrahydroxy-2,5-dimethoxyxanthone), 4 (1,3,6,8-tetrahydroxy-5-methoxyxanthone), 5 (7-methoxyluteolin-8-C--glucopyranoside), 6 (7-methoxyluteolin-6-C--glucopyranoside), 7 (7,3'-dimethoxyluteolin-6-C--glucopyranoside), 8 (6-hydroxyluteolin) and the mixture M (A-1,3,6-trihydroxy-2-methoxyxanthone and B-1,3,6-trihydroxy-2,5-dimethoxyxanthone) were evaluated for their potential to cause mutations and also to prevent the occurrence of them, using the Ames test, and it was found that all extracts and their isolated compounds were not considered mutagenic under the test conditions, and they also showed a strong to moderate antimutagenic effect against aflatoxin B1 (AFB1), benzo[a]pyrene (BaP), 4-nitro-o-phenilenodiamine (NPD), mitomycin C (MMC) and sodium azide (SA). They were also evaluated for the potential to cause DNA damage and to prevent and/or repair the damage caused by genotoxic agents, using the Comet assay, which identified the extracts Sd, Sn, Ss and the flavones 1 and 5 as causing significant DNA damage, after 4 hours of treatment. Such damage has been repaired by the cells, since no genotoxic effect was observed after 24 hours. Also Sm, Sn, Ss and all isolated compounds, except 8, were able to inhibit the damage caused by the genotoxic agent MMS. They were evaluated for their potential to cause cancer cells (HepG2 and MCF-7/BUS) death, and we found that all extracts, except Sm, and all the compounds were able to induce significant death of HepG2 cells, with most of the samples reaching 50% cell death. In MCF-7/BUS cells, after 24 hours of treatment, values were lower than 50% cell death... (Complete abstract click electronic access below)
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Palavras-chave
Estrógenos, Eriocaulacea, Células cancerosas, Cancer, Mutagenese, Estrogen
Como citar
OLIVEIRA-HÖHNE, Ana Paula. Potencial estrogênico, antimutagênico e antigenotóxico de extratos e substâncias isoladas do gênero Syngonanthus (Eriocaulaceae) e efeito inibidor na expressão de genes por xantonas de Syngonanthus nitens. 2013. 186 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Farmacêuticas, 2013.