Ação da terapia fotodinâmica no tratamento da doença periodontal experimentalmente induzida em ratos tratados ou não com nicotina: estudo histológico, histométrico e imunoistoquímico
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Data
2010-11-24
Autores
Fernandes, Leandro Araújo [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O objetivo do presente estudo foi avaliar a influência da terapia fotodinâmica (PDT) como coadjuvante ao tratamento não cirúrgico da doença periodontal (DP) experimentalmente induzida em ratos tratados ou não com nicotina. Cento e oitenta ratos foram divididos em 2 grupos de 90 animais cada. Os do Grupo Controle (C) receberam aplicações subcutâneas de soro fisiológico; os do Grupo Nicotina (N) receberam aplicações subcutâneas de nicotina. As injeções foram realizadas duas vezes ao dia e iniciadas 30 dias antes da indução da DP, continuando pós tratamento periodontal até os respectivos períodos de sacrifício. A DP foi induzida através da colocação de ligadura com fio de algodão na região dento-gengival dos primeiros molares inferiores esquerdos. Após 7 dias da indução, a ligadura foi removida e, os animais foram divididos em subgrupos de acordo com os seguintes tratamentos locais: Tratamento I - raspagem e alisamento radicular (RAR) e irrigação com soro fisiológico; Tratamento II - RAR e irrigação com azul de toluidina O (TBO) e Tratamento III – RAR, irrigação com TBO e, após 1 minuto, aplicação do laser em baixa intensidade (LLLT) (GaAsAl, 660 nm, 4J), realizando a PDT. Dez animais de cada subgrupo foram sacrificados aos 7, 15 e 30 dias. Os espécimes foram processados laboratorialmente e analisados histológica, histométrica e imunoistoquímicamente. Aos 7, 15 e 30 dias, em ambos os grupos, o tratamento RAR apresentou um tecido conjuntivo desorganizado, com elevado número de neutrófilos e discreto número de fibroblastos. O tecido ósseo apresentou áreas de necrose e trabéculas ósseas finas. Nos períodos de 7 e 15 dias os animais dos grupos C e N, tratados pela PDT, apresentaram um tecido conjuntivo bem desenvolvido com moderado número de fibroblastos, discreto infiltrado inflamatório crônico e tecido ósseo moderadamente...
The aim of this study was to evaluate the influence of photodynamic therapy (PDT) as an adjunct to non-surgical treatment of periodontal disease (PD) experimental induced in rats treated with or without nicotine. One hundred and eighty rats were divided into 2 groups of 90 animals each. Control group (C) received subcutaneous infusions of saline, group of Nicotine (N) received subcutaneous infusions of nicotine. The injections were performed twice a day and started 30 days before induction of PD, continuing after periodontal treatment until their period of sacrifice. PD was induced by placing cotton ligature in the dento-gingival region of the left lower first molars. After 7 days of induction, the ligature was removed and the animals were divided into subgroups according to the following local treatments: treatment I - scaling and root planing (SRP) and irrigation with saline; Treatment II - SRP and irrigation with O toluidine blue (TBO) and Treatment III - SRP, irrigation with TBO and 1 minute after application of low intensity laser therapy (LLLT) (GaAlAs, 660 nm, 4J), performing the PDT. Ten animals of each subgroup were sacrificed at 7, 15 and 30 days. The laboratory specimens were processed and analyzed histologically, histometrically and immunohistochemically. At 7, 15 and 30 days in both groups, treatment SRP showed a disorganized tissue with high numbers of neutrophils and a small number of fibroblasts. The bone tissue showed areas of necrosis and trabecular bone thin. At 7 and 15 days the animals in groups C and N, treated by PDT, showed a well developed connective tissue with a moderate number of fibroblasts, mild chronic inflammatory infiltrate and bone moderately developed. At 30 days, they presented an intact periodontal ligament, and organized collagen fibers in parallel. The connective tissue was well-developed, healthy and with no inflammatory... (Complete abstract click electronic access below)
The aim of this study was to evaluate the influence of photodynamic therapy (PDT) as an adjunct to non-surgical treatment of periodontal disease (PD) experimental induced in rats treated with or without nicotine. One hundred and eighty rats were divided into 2 groups of 90 animals each. Control group (C) received subcutaneous infusions of saline, group of Nicotine (N) received subcutaneous infusions of nicotine. The injections were performed twice a day and started 30 days before induction of PD, continuing after periodontal treatment until their period of sacrifice. PD was induced by placing cotton ligature in the dento-gingival region of the left lower first molars. After 7 days of induction, the ligature was removed and the animals were divided into subgroups according to the following local treatments: treatment I - scaling and root planing (SRP) and irrigation with saline; Treatment II - SRP and irrigation with O toluidine blue (TBO) and Treatment III - SRP, irrigation with TBO and 1 minute after application of low intensity laser therapy (LLLT) (GaAlAs, 660 nm, 4J), performing the PDT. Ten animals of each subgroup were sacrificed at 7, 15 and 30 days. The laboratory specimens were processed and analyzed histologically, histometrically and immunohistochemically. At 7, 15 and 30 days in both groups, treatment SRP showed a disorganized tissue with high numbers of neutrophils and a small number of fibroblasts. The bone tissue showed areas of necrosis and trabecular bone thin. At 7 and 15 days the animals in groups C and N, treated by PDT, showed a well developed connective tissue with a moderate number of fibroblasts, mild chronic inflammatory infiltrate and bone moderately developed. At 30 days, they presented an intact periodontal ligament, and organized collagen fibers in parallel. The connective tissue was well-developed, healthy and with no inflammatory... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Fotoquimioterapia, Lasers, Nicotina, Perda óssea alveolar, Periodontite, Osteoclastos, Photochemotherapy, Nicotine, Osteoclasts, Alveolar bone loss, Periodontitis
Como citar
FERNANDES, Leandro Araújo. Ação da terapia fotodinâmica no tratamento da doença periodontal experimentalmente induzida em ratos tratados ou não com nicotina: estudo histológico, histométrico e imunoistoquímico. 2010. 118 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araçatuba, 2010.