Avaliação do efeito da Sinvastatina em ratos submetidos a periodontite experimental e imunossuprimidos por Ciclosporina-A e Tacrolimus
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Data
2008-03-27
Autores
Nassar, Patrícia Oehlmeyer [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Há relatos na literatura que os ossos de maneira geral assim como o processo alveolar são acometidos pela Ciclosporina-A (CsA) desequilibrando sua homeostase, induzindo a perda óssea. Alguns trabalhos experimentais mostram que o Tacrolimus (FK506) induz perda óssea, enquanto outros relatam conferir uma proteção contra inflamação e perda óssea associada à periodontite, em ratos com doença periodontal induzida. Adicionalmente, tem sido recentemente revelado que algumas estatinas poderiam estimular a formação óssea e inibir a reabsorção óssea. Assim os objetivos do estudo foram avaliar os efeitos da administração da CsA, FK506 associados ou não com sinvastatina no desenvolvimento da doença periodontal induzida em ratos submetidos a modelo de periodontite experimental, através de avaliações bioquímica, molecular e esterométrica. Foram utilizados 320 ratos divididos em 32 grupos e em diferentes períodos de 15, 30 e 60 dias associados ou não a periodontite experimental e/ou a administração diária de CsA (10mg/kg por peso corporal), FK506 (1mg/kg por peso corporal ) e Sinvastatina (20mg/kg por peso corporal). Ao final de cada período experimental, coletas de sangue foram realizadas para medir os níveis séricos de cálcio, fósforo e fosfatase alcalina e biópsias gengivais para as avaliações de ELISA e PCR. Após o processamento histológico, a densidade volumétrica de osso, osteoblastos e osteoclastos foram medidas na região de primeiro molar inferior. Após 15 e 30 dias de tratamento quando associado ao uso de Sinvastatina, houve melhora nos parâmetros bioquímicos em relação à CsA (p<0.05) e sem ser significante em relação ao FK506 (p>0.05) com ou sem associação de doença periodontal.
In the literature that the bones as well as the alveolar process are attack by the CsA having unbalanced its homeostasis, inducing the bone loss. Some experimental studies show that the FK506 induced bone loss, while others demonstrated to confer a protection against inflammation and bone loss associate to the periodontitis, in rats with induced periodontal disease. Additionally, he has been recently disclosed that some statins could stimulate the bone formation and inhibit the bone resorption. Thus the objectives of the study had been to evaluate the effect of the administration of the CsA, FK506 associates or not with sinvastatin in the development of the induced periodontal disease in submitted rats the model of experimental periodontite, through evaluations biochemistry, molecular and stereometric. 320 rats divided in 32 groups and different periods of 15, 30 and 60 days had been used associates or not to the experimental periodontitis and/or the daily administration of CsA (10mg/kg body weight), FK506 (1mg/kg body weight) and Sinvastatin (20mg/kg body weight). At the end of period experimental, blood collection was obtained and serum levels calcium, phosphorus and alkaline phosphatase were measured and ELISA end PCR analysis were extracted from gingival tissue samples. After the histologic processing, the volumetric density of bone, osteoblasts and osteoclasts had been measured in the first region molar inferior. After 15 and 30 days of treatment when associated to the use of sinvastatin, it had improvement in the parameters biochemistries in relation to the CsA (p<0,05) with or without association of periodontal disease The same characteristics had been presented in the stereometric analysis.
In the literature that the bones as well as the alveolar process are attack by the CsA having unbalanced its homeostasis, inducing the bone loss. Some experimental studies show that the FK506 induced bone loss, while others demonstrated to confer a protection against inflammation and bone loss associate to the periodontitis, in rats with induced periodontal disease. Additionally, he has been recently disclosed that some statins could stimulate the bone formation and inhibit the bone resorption. Thus the objectives of the study had been to evaluate the effect of the administration of the CsA, FK506 associates or not with sinvastatin in the development of the induced periodontal disease in submitted rats the model of experimental periodontite, through evaluations biochemistry, molecular and stereometric. 320 rats divided in 32 groups and different periods of 15, 30 and 60 days had been used associates or not to the experimental periodontitis and/or the daily administration of CsA (10mg/kg body weight), FK506 (1mg/kg body weight) and Sinvastatin (20mg/kg body weight). At the end of period experimental, blood collection was obtained and serum levels calcium, phosphorus and alkaline phosphatase were measured and ELISA end PCR analysis were extracted from gingival tissue samples. After the histologic processing, the volumetric density of bone, osteoblasts and osteoclasts had been measured in the first region molar inferior. After 15 and 30 days of treatment when associated to the use of sinvastatin, it had improvement in the parameters biochemistries in relation to the CsA (p<0,05) with or without association of periodontal disease The same characteristics had been presented in the stereometric analysis.
Descrição
Palavras-chave
Doenças periodontais, Ciclosporina, Tacrolimo, Sinvastatina, Tacrolimus, Periodontal diseases, Cyclosporin, Sinvastatin
Como citar
NASSAR, Patrícia Oehlmeyer. Avaliação do efeito da Sinvastatina em ratos submetidos a periodontite experimental e imunossuprimidos por Ciclosporina-A e Tacrolimus. 2008. 116 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araraquara, 2008.