Caracterização e ocorrência de bidens mosaic virus (bimv) em regiões produtoras de alface no Estado de São Paulo
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Data
2009-12-15
Autores
Sanches, Márcio Martinello [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O Bidens mosaic virus (BiMV) é uma espécie tentativa de vírus do gênero Potyvirus e tem sido encontrado em culturas de importância econômica como ervilha e girassol. Recentemente foi proposto que o BiMV poderia ser uma estirpe do Potato virus Y (PVY). Em 2004 amostras de alface apresentando mosaico intenso, coletadas no município de São Manuel - SP, apresentavam-se infectadas pelo BiMV. Foi realizada a caracterização biológica, o sequenciamento parcial e a purificação deste isolado para obtenção de anti-soro policlonal. Verificou-se que este isolado possui gama de hospedeiros restrita à alface, Chenopodium quinoa, Chenopodium amaranticolor e ervilha (Pisum sativum) de forma assintomática. O vírus possui uma proteína capsidial em torno de 33 kDa e padronizou-se um teste de PTAELISA para o diagnóstico sorológico deste vírus. Também foi realizada a caracterização biológica e avanços no sequenciamento do genoma de um isolado de BiMV coletado a partir da planta de Bidens pilosa (picão preto). Este isolado foi capaz de infectar e causar sintomas em girassol (Helianthus annus), alface (Lactuca sativa), ervilha (Pisum sativum), C. quinoa, C. amaranticolor, Nicotiana benthamiana, N. clevelandii, N. occidentalis, Zinnia elegans e em Gomphrena globosa, nesta última de forma assintomática. Através do sequenciamento de um fragmento de 7.945 bp, correspondente à parte do gene da HC-Pro e dos genes da P3, 6K1, CI, 6K2, VPg, NIa, NIb, CP e à região 3’UTR, verificou-se que o BiMV é possivelmente uma espécie distinta do PVY, baseando-se nos atuais critérios para demarcação de espécies do International Committee on Taxonomy of Viruses (ICTV). Foram obtidos oligonucleotídeos específicos para diagnose do BiMV, que através de RT-PCR em uma só etapa, detectam eficientemente o vírus a partir de alface e de plantas daninhas associadas à cultura. Através desta técnica...
Bidens mosaic virus (BiMV) is a tentative species of the genus Potyvirus, and has been commonly found infecting pea and sunflower. Recently it was proposed that BiMV could be a member of the Potato virus Y (PVY) species. In 2004 lettuce plants showing intense mosaic symptoms were collected in São Manuel-SP and were infected with BiMV. The host range of this isolate was characterized, the genome partially sequenced and the virus was purified to obtain a polyclonal antiserum. The host range for this isolate was restricted to lettuce, Chenopodium quinoa, Chenopodium amaranticolor and pea (Pisum sativum) (assymptomatic). The virus has a coat protein with approximately 33 kDa and the antiserum could be used for serological diagnosis by PTA-ELISA tests. A BiMV isolate from Bidens pilosa was also studied in this work. This isolate infected sunflower (Helianthus annus), lettuce (Lactuca sativa), pea (Pisum sativum), C.quinoa, C. amaranticolor, N. benthamiana, N. clevelandii, N. occidentalis, Zinnia elegans and Gomphrena globosa (assymptomatic). A fragment of 7.945bp including part of the HC-Pro, the entire genes for P3, 6K1, CI, 6K2, VPg, NIa, Nib, CP and the 3’UTR region was sequenced. Based on the International Committee on Taxonomy of Viruses (ICTV) criteria of potyvirus species demarcation using the individual genes, BiMV could be considered a distinct species of PVY. Specific primers were obtained for BiMV diagnosis and efficiently used in a one step RT-PCR test for BiMV diagnoses. A total of 222 lettuce and 126 weeds samples were collected in the lettuce growing areas of Mogi das Cruzes, Campinas and Bauru and analysed by RT-PCR. Low incidence of BiMV was verified on lettuce. The presence of BiMV was also observed on B. pilosa and Galinsoga parviflora. This last plant species was identified as a new host of BiMV and Lettuce mottle virus (LeMoV) in this study.
Bidens mosaic virus (BiMV) is a tentative species of the genus Potyvirus, and has been commonly found infecting pea and sunflower. Recently it was proposed that BiMV could be a member of the Potato virus Y (PVY) species. In 2004 lettuce plants showing intense mosaic symptoms were collected in São Manuel-SP and were infected with BiMV. The host range of this isolate was characterized, the genome partially sequenced and the virus was purified to obtain a polyclonal antiserum. The host range for this isolate was restricted to lettuce, Chenopodium quinoa, Chenopodium amaranticolor and pea (Pisum sativum) (assymptomatic). The virus has a coat protein with approximately 33 kDa and the antiserum could be used for serological diagnosis by PTA-ELISA tests. A BiMV isolate from Bidens pilosa was also studied in this work. This isolate infected sunflower (Helianthus annus), lettuce (Lactuca sativa), pea (Pisum sativum), C.quinoa, C. amaranticolor, N. benthamiana, N. clevelandii, N. occidentalis, Zinnia elegans and Gomphrena globosa (assymptomatic). A fragment of 7.945bp including part of the HC-Pro, the entire genes for P3, 6K1, CI, 6K2, VPg, NIa, Nib, CP and the 3’UTR region was sequenced. Based on the International Committee on Taxonomy of Viruses (ICTV) criteria of potyvirus species demarcation using the individual genes, BiMV could be considered a distinct species of PVY. Specific primers were obtained for BiMV diagnosis and efficiently used in a one step RT-PCR test for BiMV diagnoses. A total of 222 lettuce and 126 weeds samples were collected in the lettuce growing areas of Mogi das Cruzes, Campinas and Bauru and analysed by RT-PCR. Low incidence of BiMV was verified on lettuce. The presence of BiMV was also observed on B. pilosa and Galinsoga parviflora. This last plant species was identified as a new host of BiMV and Lettuce mottle virus (LeMoV) in this study.
Descrição
Palavras-chave
Virus de plantas, Alface, Potyvirus, BiMV, Galinsoga parviflora. eng, Bidens pilosa, Lactuca sativa
Como citar
SANCHES, Márcio Martinello. Caracterização e ocorrência de bidens mosaic virus (bimv) em regiões produtoras de alface no Estado de São Paulo. 2009. xi, 107 f. Tese (doutorado) - Universidade Estadual Paulista , Faculdade de Ciências Agronômicas de Botucatu, 2009.