Avaliação da proteção renal de doses altas de melatonina em modelo de experimentação de isquemia e reperfusão renal em ratos submetidos à hiperglicemia
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Data
2013-08-27
Autores
Souza, Aparecida Vitória Gonçalves de [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
A melatonina é um varredor de radicais livres com importantes ações no estudo da isquemia e reperfusão renal. O objetivo deste estudo é avaliar possível proteção renal de altas doses de melatonina em modelo experimental de I/R submetidos à hiperglicemia aguda em ratos anestesiados com isoflurano. Método: utilizou-se 44 ratos Wistar, machos, com peso superior a 300g, distribuídos de modo aleatório em cinco grupos, designados: sham (n=10); melatonina, (n=10, 50 mg.kg- 1);hiperglicemia (n=9, glicose 2,5 g.kg-1); hiperglicemia/melatonina (n=10, glicose 2,5 g.kg-1 + melatonina 50 mg.kg-1); isquemia e reperfusão (n=5,). Todos os grupos receberam indução da anestesia com isoflurano a 4% e a manutenção com isoflurano de 1,5 a 2,0%. A pressão arterial média (PAM) foi medida para controle da anestesia. A injeção intraperitoneal da melatonina (melatonina e hiperglicemia/melatonina) ou glicose (hiperglicemia, hiperglicemia/melatonina) ou solução salina (isquemia e reperfusão) foi feita 40 minutos antes da isquemia renal esquerda. Nos três grupos a isquemia durou 25 minutos. Todos os animais foram submetidos à nefrectomia direita. Os valores plasmáticos da creatinina e glicose foram determinados no início (M1), no (M2) imediatamente após reperfusão e 24 horas após o final do experimento (M3), quando os animais retornaram ao laboratório e foram anestesiados com isoflurano para coleta de amostra sanguínea e nefrectomia esquerda. Para análise histológica foi utilizada uma escala para avaliação da necrose tubular (0 a 5 = lesão máxima). Houve tratamento estatístico para os valores da temperatura dos animais, peso, PAM, da creatinina plasmática, glicemia e das lesões histológicas, sendo as diferenças consideradas significantes quando P < 0,05. A glicemia foi superior no M2 nos grupos que receberam suplementação de glicose, hiperglicemia (356,00 ± 107,83) e hiperglicemia/melatonina...
Melatonin is a free radical scavenger with important actions in the study of renal ischemia and reperfusion (I/R). This study aimed evaluate possible renal protection of high doses of melatonin in an experimental model of I/R, in which rats were submitted to acute hyperglycemia under anesthesia with isoflurane. Method: 44 male Wistar rats, weighing more than 300g, were randomly divided into five groups: sham (n=10); melatonin (n=10, 50 mg.kg-1); hyperglycemia (n=9, glucose 2.5 g.kg-1); hyperglycemia/melatonin (n=10, 2.5 g.kg-1 glucose + melatonin 50 mg.kg-1); ischemia and reperfusion (n=5). In all groups, anesthesia was induced with 4% isoflurane and maintained with 1.5 to 2.0% isoflurane. Mean arterial pressure (MAP) was measured to control anesthesia. Intraperitoneal injection of melatonin (melatonin and hyperglycemia/melatonin), glucose (hyperglycemia, hyperglycemia/melatonin) or saline (ischemia and reperfusion) was performed 40 min before left renal ischemia. In these groups, ischemia lasted 25 min. All the rats were submitted to right nephrectomy. Serum plasma values for creatinine and glucose were determined at baseline (T1), immediately following reperfusion (T2) and 24 h after completion of the experiment (T3), when the rats were returned to the laboratory and anesthetized with isoflurane to collect blood samples and for left nephrectomy. Histological analysis was performed to evaluate tubular necrosis (scale: 0 to 5 = maximal lesion). Statistical analysis was applied to the following values: temperature, weight, MAP, plasma creatinine, glucose and histological lesions. The differences were considered significant when P <0.05. Serum glucose was higher at T2 in the groups supplemented with glucose, hyperglycemia (356.00 ±107.83) and hyperglycemia/melatonin (445.3 ±148.32). Creatinine values were higher at T3 (P=0.0001) for ischemia and reperfusion (3.6 ±0.37), hyperglycemia/melatonin ...
Melatonin is a free radical scavenger with important actions in the study of renal ischemia and reperfusion (I/R). This study aimed evaluate possible renal protection of high doses of melatonin in an experimental model of I/R, in which rats were submitted to acute hyperglycemia under anesthesia with isoflurane. Method: 44 male Wistar rats, weighing more than 300g, were randomly divided into five groups: sham (n=10); melatonin (n=10, 50 mg.kg-1); hyperglycemia (n=9, glucose 2.5 g.kg-1); hyperglycemia/melatonin (n=10, 2.5 g.kg-1 glucose + melatonin 50 mg.kg-1); ischemia and reperfusion (n=5). In all groups, anesthesia was induced with 4% isoflurane and maintained with 1.5 to 2.0% isoflurane. Mean arterial pressure (MAP) was measured to control anesthesia. Intraperitoneal injection of melatonin (melatonin and hyperglycemia/melatonin), glucose (hyperglycemia, hyperglycemia/melatonin) or saline (ischemia and reperfusion) was performed 40 min before left renal ischemia. In these groups, ischemia lasted 25 min. All the rats were submitted to right nephrectomy. Serum plasma values for creatinine and glucose were determined at baseline (T1), immediately following reperfusion (T2) and 24 h after completion of the experiment (T3), when the rats were returned to the laboratory and anesthetized with isoflurane to collect blood samples and for left nephrectomy. Histological analysis was performed to evaluate tubular necrosis (scale: 0 to 5 = maximal lesion). Statistical analysis was applied to the following values: temperature, weight, MAP, plasma creatinine, glucose and histological lesions. The differences were considered significant when P <0.05. Serum glucose was higher at T2 in the groups supplemented with glucose, hyperglycemia (356.00 ±107.83) and hyperglycemia/melatonin (445.3 ±148.32). Creatinine values were higher at T3 (P=0.0001) for ischemia and reperfusion (3.6 ±0.37), hyperglycemia/melatonin ...
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Palavras-chave
Hiperglicemia, Melatonina, Rins - Efeito das drogas - Estudos experimentais, Isquemia, Antioxidantes, Kidneys
Como citar
SOUZA, Aparecida Vitória Gonçalves de. Avaliação da proteção renal de doses altas de melatonina em modelo de experimentação de isquemia e reperfusão renal em ratos submetidos à hiperglicemia. 2013. 53 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina de Botucatu, 2013.