Publicação: Penicillinase-based amperometric biosensor for penicillin G
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Editor
Elsevier B.V.
Tipo
Artigo
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Resumo
A biosensor for penicillin G was created by immobilizing penicillinase to a gold electrode by means of a cysteine self-assembled monolayer. The biosensor amperometrically monitored the catalytic hydrolysis of penicillin in a very sensible manner. Furthermore, it was successfully used to measure the Michaelis-Menten enzymatic constant and a low limit of detection of 4.5 nM was obtained. (C) 2013 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
Cysteine, Metallo-beta-lactamase, Self-assembled monolayer (SAM), beta-Lactam antibiotic, Lineweaver-Burk plot
Idioma
Inglês
Como citar
Electrochemistry Communications. New York: Elsevier Science Inc, v. 38, p. 131-133, 2014.
