Penicillinase-based amperometric biosensor for penicillin G
Nenhuma Miniatura disponível
Data
2014-01-01
Autores
Goncalves, Luis Moreira
Callera, Welder F. A. [UNESP]
Sotomayor, Maria Del Pilar Taboada [UNESP]
Bueno, Paulo Roberto [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
A biosensor for penicillin G was created by immobilizing penicillinase to a gold electrode by means of a cysteine self-assembled monolayer. The biosensor amperometrically monitored the catalytic hydrolysis of penicillin in a very sensible manner. Furthermore, it was successfully used to measure the Michaelis-Menten enzymatic constant and a low limit of detection of 4.5 nM was obtained. (C) 2013 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
Cysteine, Metallo-beta-lactamase, Self-assembled monolayer (SAM), beta-Lactam antibiotic, Lineweaver-Burk plot
Como citar
Electrochemistry Communications. New York: Elsevier Science Inc, v. 38, p. 131-133, 2014.