Chilling curves for Piaractus mesopotamicus (Holmberg, 1887) embryos stored at-8 degrees C
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Data
2013-11-01
Autores
Lopes, Tais da S. [UNESP]
Streit, Danilo Pedro
Fornari, Darci Carlos
Oliveira, Diego de
Ribeiro, Ricardo Pereira
Romagosa, Elizabeth
Título da Revista
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Título de Volume
Editor
Cambridge University Press
Resumo
The present study investigates the effect of different slow chilling curves on the storage of pacu (Piaractus mesopotamicus) embryos submitted to chilling at -8 degrees C. Embryos at the blastopore closure stage were divided into two groups: G1 - embryos exposed to cryoprotectant solution containing methanol (10%) and sucrose (0.5M), treated as follows: (T1) taken directly from room temperature to the refrigerator without being submitted to the curve; (T2) chilling curve of 0.5 degrees C/min; and (T3) chilling curve of 1 degrees C/min; and G2 - the cryoprotectant solution alone was submitted to these same temperatures, receiving the embryos only after temperature had decreased, corresponding to treatments T4, T5 and T6, respectively. Treatments were kept at -8 degrees C for a period of 6 h. Embryo development was evaluated for each treatment, with six replicates in an entirely randomized design. Survival among embryos not submitted to refrigeration was 94.3 +/- 8.05%. Percentage of total larvae (TL) and addled eggs (AE) did not differ statistically between the groups, although percentage of swimming larvae (SL) exhibited higher values in G1 for the 1 degrees C/min curve. Furthermore, when comparing the three chilling curves, a decrease of 1 degrees C/min resulted in the highest TL percentage (90.85%), followed by the 0.5.C/min curve (78.52%). Thus, the use of 1 degrees C/min chilling curves is recommended for P. mesopotamicus embryos stored for 6 h at -8 degrees C.
Descrição
Palavras-chave
Cryopreservation, Embryo chilling, Larvae survival, Pacu
Como citar
Zygote. New York: Cambridge Univ Press, v. 21, n. 4, p. 345-350, 2013.