Effects of Helicobacter pylori Infection on the Expressions of Bax and Bcl-2 in Patients with Chronic Gastritis and Gastric Cancer
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Data
2010-01-01
Autores
Bartchewsky, Waldemar
Martini, Mariana R.
Squassoni, Aline C.
Alvarez, Marisa C.
Ladeira, Marcelo S. P. [UNESP]
Salvadori, Daisy Maria Favero [UNESP]
Trevisan, Miriam A.
Pedrazzoli, Jose
Ribeiro, Marcelo L.
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Editor
Springer
Resumo
The aim of the present study is to evaluate the influence of Helicobacter pylori on Bax and Bcl-2 mRNA and protein levels in patients with chronic gastritis and gastric cancer. The study included 217 patients, of which 26 were uninfected; 127 had chronic gastritis and were H. pylori-positive, and 64 had gastric cancer. Bacterial genotypes were evaluated by PCR, and the expression values were determined by quantitative real-time PCR and immunohistochemistry. Our data showed that the up-regulationary effects of H. pylori infection on the proapoptotic gene, Bax, were stronger than its induction of Bcl-2; this effect may increase apoptosis in patients with chronic gastritis. In patients with gastric cancer, the up-regulation of the anti-apoptotic gene, Bcl-2, counteracted the pro-apoptotic effects of Bax, leading to a deregulation of apoptosis-associated gene expression, favoring cell proliferation. Thus, the disturbance in Bax and Bcl-2 balance, induced by H. pylori, might be important in gastric cancer development.
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Palavras-chave
Helicobacter pylori, Gastric cancer, Bax, Bcl-2, Real-time PCR
Como citar
Digestive Diseases and Sciences. Dordrecht: Springer, v. 55, n. 1, p. 111-116, 2010.