Phage-displayed peptides as capture antigens in an innovative assay for Taenia saginata-infected cattle
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Data
2014-11-01
Autores
Fogaca, Rafaela L.
Capelli-Peixoto, Janaina
Yamanaka, Isabel B.
Almeida, Rodrigo P. M. de
Muzzi, Joao Carlos D.
Borges, Mariangela
Costa, Alvimar J. [UNESP]
Chavez-Olortegui, Carlos
Thomaz-Soccol, Vanete
Alvarenga, Larissa M.
Título da Revista
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Título de Volume
Editor
Springer
Resumo
Bovine cysticercosis is detected during the routine post mortem examination of carcasses by visual inspection (knife and eye method). However, the sensitivity of this procedure is several times lower than immunoassays, even when it is performed by qualified professionals. In the present study, a new generation capture antigens were screened from a phage display peptide library using antibodies from Taenia saginata-infected animals. Eight phage clones were selected, and one, Tsag 3 (VHTSIRPRCQPRAITPR), produced similar results to the T. saginata metacestode crude antigen (TsCa) when used as a capture antigen in an ELISA. The phage-displayed peptides competed with TsCa for binding sites, reducing the reactivity by approximately 30 %. Alanine scanning indicated that proline, arginine, and serine are important residues for antibody binding. Tsag 1 (HFYQITWLPNTFPAR), the most frequent affinity-selected clone, and Tsag 6 (YRWPSTPSASRQATL) shared similarity with highly conserved proteins from the Taeniidae family with known immunogenicity. Due to their epitopic or mimotopic properties, these affinity-selected phages could contribute to the rational design of an ante mortem immunodiagnosis method for bovine cysticercosis, as well as an epitope-based vaccine to interrupt the taeniosis/cysticercosis complex.
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Palavras-chave
Bovine cysticercosis, Immunodiagnosis, Phage-displayed peptide, Taenia saginata
Como citar
Applied Microbiology And Biotechnology. New York: Springer, v. 98, n. 21, p. 8887-8894, 2014.