Immunohistochemical and molecular analysis of caveolin-1 expression in canine mammary tumors
Carregando...
Arquivos
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Funpec-editora
Tipo
Artigo
Direito de acesso
Acesso aberto
Resumo
Caveolin-1 (Cav-1) is a structural protein present in invaginations of the cell membrane. In human breast cancer, the cav-1 gene is believed to be a tumor suppressor gene associated with inhibition of tumor metastasis. However, little is known about its expression, regulation and function in canine mammary tumors. Expression levels of cav-1 were investigated using real- time PCR and immunohistochemical detection with an anti-human Cav- 1 antibody. Gene expression stability of different samples was analyzed using the geNorm software. Mammary tumors from 51 female dogs were compared to normal mammary tissue from 10 female dogs. Malignant mammary cells showed a loss of Cav-1 expression by quantitative RTPCR and weak Cav-1 staining by immunohistochemistry compared to normal mammary gland tissue. There was a significant relationship between outcome and immunostaining as well as with tumor size, indicating that caveolin subexpression has a positive predictive value and is related to higher survival and smaller tumor size. Our findings indicate that Cav-1 is a potential prognostic marker for canine mammary tumors.
Descrição
Palavras-chave
Mammary cancer, Prognosis, Immunohistochemistry, Quantitative PCR
Idioma
Inglês
Citação
Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 11, n. 1, p. 153-165, 2012.
