MICROPROPAGAÇÃO do KIWI CV. HAYWARD
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Data
2001-12-01
Autores
SCALIZE, FÁBIO EDUARDO [UNESP]
Moro, Fabiola Vitti [UNESP]
DAMIÃO FILHO, CARLOS FERREIRA [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Fruticultura
Resumo
O presente trabalho teve como objetivo desenvolver um protocolo para a obtenção de mudas de kiwi (Actinidia chinensis Planch), cv. Hayward, por meio do cultivo in vitro de cotilédones. Utilizou-se o meio de MURASHIGE & SKOOG (1962) -- MS, suplementado com dois tipos de auxina (AIA e AIB) e uma citocinina (BAP). Foram verificados os efeitos de três doses de auxinas (0,125; 0,250 e 0,375 mg.L-1), combinadas com três doses de citocinina (0,5; 1,0 e 1,5 mg.L-1) na capacidade morfogênica dos explantes. Procedeu-se o estudo histológico dos órgãos das plântulas obtidas in vitro, e verificou-se, também, a capacidade de aclimatação das mudas ex vitro. A menor dose de AIB (0,125 mg.L-1), independentemente das doses de BAP, foi a mais eficaz na morfogênese dos explantes. Não foram verificadas alterações histológicas e anatômicas das plântulas obtidas in vitro. Aos três meses após o cultivo ex vitro dos explantes, verificou-se a sobrevivência de 88% das plantas transplantadas em condições de campo.
The aim of this paper was to develop a protocol for plantlets production in kiwi fruit (Actinidia chinensis Planch) Hayward through in vitro cotyledons cultivation. For this purpose the Murashige & Skoog (1962) medium was supplemented with two types of auxin (IAA and IBA) and also with a cytokinin (BAP). Effects over the morphogenic capacity of the explants were tested using three auxin (0.125; 0.250 and 0.375 mg.L-1) and cytokinin (0.5; 1.0 and 1.5 mg.L-1) concentrations. Studies in the obtained plantlets histology and acclimatization capacity were proceeded. It could be concluded that IBA at the lowest concentration (0.125 mg. L-1) was the most effective in explants morphogenesis, regardless of the BAP concentration. In vitro obtained plantlets did not present any anatomical nor histological alterations. Three months after transplanting it was observed that 88% of the plantlets had survived at field conditions.
The aim of this paper was to develop a protocol for plantlets production in kiwi fruit (Actinidia chinensis Planch) Hayward through in vitro cotyledons cultivation. For this purpose the Murashige & Skoog (1962) medium was supplemented with two types of auxin (IAA and IBA) and also with a cytokinin (BAP). Effects over the morphogenic capacity of the explants were tested using three auxin (0.125; 0.250 and 0.375 mg.L-1) and cytokinin (0.5; 1.0 and 1.5 mg.L-1) concentrations. Studies in the obtained plantlets histology and acclimatization capacity were proceeded. It could be concluded that IBA at the lowest concentration (0.125 mg. L-1) was the most effective in explants morphogenesis, regardless of the BAP concentration. In vitro obtained plantlets did not present any anatomical nor histological alterations. Three months after transplanting it was observed that 88% of the plantlets had survived at field conditions.
Descrição
Palavras-chave
micropropagação, kiwi, cotilédones, aclimatação ex vitro, micropropagation, kiwi, cotyledons, ex vitro acclimatization
Como citar
Revista Brasileira de Fruticultura. Sociedade Brasileira de Fruticultura, v. 23, n. 3, p. 656-661, 2001.