DNA damage in peripheral blood mononuclear cells of patients undergoing anti-tuberculosis treatment
Nenhuma Miniatura disponível
Data
2012-08-30
Autores
Cardoso de Oliveira, Larissa Ragozo [UNESP]
Peresi, Eliana [UNESP]
Tavares, Francilene Capel [UNESP]
Corrêa, Camila Renata [UNESP]
Pierine, Damiana Tortolero [UNESP]
Calvi, Sueli Aparecida [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Tuberculosis (TB), a chronic infectious disease, is a major cause of morbidity and mortality worldwide. Expression of iNOS and consequent production of NO during the inflammatory process is an important defense mechanism against TB bacteria. We have tested whether pulmonary TB patients undergoing anti-tuberculosis treatment present DNA damage, and whether this damage is related to oxidative stress, by evaluating total hydrophilic antioxidant capacity and iNOS expression. DNA damage in peripheral blood mononuclear cells from patients and healthy tuberculin test (PPD) positive controls was evaluated by single-cell gel electrophoresis (comet assay), and iNOS expression was measured by qPCR. We also evaluated total hydrophilic antioxidant capacity in plasma from patients and controls. Compared to controls, pulmonary TB patients under treatment presented increased DNA damage, which diminished during treatment. Also, the antioxidant capacity of these individuals was increased at the start of treatment, and reduced during treatment. TB patients showed lower iNOS expression, but expression tended to increase during treatment. Our results indicate that pulmonary TB patients under anti-TB treatment exhibit elevated DNA damage in peripheral blood mononuclear cells. This damage was not related to nitric oxide but may be due to other free radicals. (C) 2012 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
DNA damage, Tuberculosis, Antioxidants, iNOS
Como citar
Mutation Research-genetic Toxicology and Environmental Mutagenesis. Amsterdam: Elsevier B.V., v. 747, n. 1, p. 82-85, 2012.