Publication: Typing class I HLA-A gene using a nested PCR-RFLP procedure
Loading...
Date
Advisor
Coadvisor
Graduate program
Undergraduate course
Journal Title
Journal ISSN
Volume Title
Publisher
Associação Brasileira de Divulgação Científica (ABRADIC)
Type
Article
Access right
Acesso aberto
Abstract
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
Description
Keywords
Human leukocyte antigen-A, HLA-A, Typing, RFLP, Polymorphism, Major histocompatibility complex
Language
English
Citation
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 6, p. 837-842, 2005.
