A step to the gigantic genome of the desert locust: chromosome sizes and repeated DNAs
Nenhuma Miniatura disponível
Data
2015-06-01
Autores
Camacho, J. P. M.
Ruiz-Ruano, F. J.
Martin-Blazquez, R.
Lopez-Leon, M. D.
Cabrero, J.
Lorite, P.
Cabral-de-Mello, D. C. [UNESP]
Bakkali, M.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Springer
Resumo
The desert locust (Schistocerca gregaria) has been used as material for numerous cytogenetic studies. Its genome size is estimated to be 8.55 Gb of DNA comprised in 11 autosomes and the X chromosome. Its X0/XX sex chromosome determinism therefore results in females having 24 chromosomes whereas males have 23. Surprisingly, little is known about the DNA content of this locust's huge chromosomes. Here, we use the Feulgen Image Analysis Densitometry and C-banding techniques to respectively estimate the DNA quantity and heterochromatin content of each chromosome. We also identify three satellite DNAs using both restriction endonucleases and next-generation sequencing. We then use fluorescent in situ hybridization to determine the chromosomal location of these satellite DNAs as well as that of six tandem repeat DNA gene families. The combination of the results obtained in this work allows distinguishing between the different chromosomes not only by size, but also by the kind of repetitive DNAs that they contain. The recent publication of the draft genome of the migratory locust (Locusta migratoria), the largest animal genome hitherto sequenced, invites for sequencing even larger genomes. S. gregaria is a pest that causes high economic losses. It is thus among the primary candidates for genome sequencing. But this species genome is about 50 % larger than that of L. migratoria, and although next-generation sequencing currently allows sequencing large genomes, sequencing it would mean a greater challenge. The chromosome sizes and markers provided here should not only help planning the sequencing project and guide the assembly but would also facilitate assigning assembled linkage groups to actual chromosomes.
Descrição
Palavras-chave
Como citar
Chromosoma. New York: Springer, v. 124, n. 2, p. 263-275, 2015.