A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
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Data
2015-05-01
Autores
Angelini, Leticia Mara Lima [UNESP]
Silva, Amanda Ribeiro Martins da [UNESP]
Rocco, Lucas de Freitas Coli [UNESP]
Milagre, Cintia Duarte de Freitas [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Soc Brasileira Microbiologia
Resumo
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
Descrição
Palavras-chave
Nitrile hydratase, Amidase, Nitrilase, Amidase inhibitor, High-throughput screening
Como citar
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 46, n. 1, p. 113-116, 2015.