Diazepam, em dose única, inibe a migração celular, a estimulação macrofágica e a atividade de TNF-α na reação inflamatória aguda induzida por LPS em camundongos
Carregando...
Arquivos
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade de São Paulo (USP), Conjunto Quimicas
Tipo
Artigo
Direito de acesso
Acesso aberto
Resumo
Benzodiazepines are one of the most frequently prescribed drugs due to their anxiolytic properties. The aim of this study was to evaluate the effects of diazepam on lipopolysaccharide-induced peritoneal acute inflammatory responses. Swiss mice were treated with diazepam in a single dose of 1 or 10 mg/kg- subcutaneously 1 h before an intraperitoneal injection of lipopolysaccharide or sterile saline solution. The mice were killed 16 h after and the cells were washed from the peritoneal cavity to determine the total number of cells and the mononuclear and polimorfonuclear subpopulations, as well as the TNF-alpha activity and percentage of spread macrophages. Our results showed that the diazepam treatment (1 and 10 mg/kg) induced a significant reduction in the LPS-induced macrophage stimulation and TNF-α activity. Diazepam (10 mg/kg) also reduced the inflammatory cellular migration when compared to the control. It can be concluded that the diazepam treatment in a single dose is able to influence the inflammatory cellular influx, macrophage stimulation and TNF-α activity in the acute inflammatory response in mice, having possible implications on the anti-infectious response efficiency.
Descrição
Palavras-chave
Acute inflammatory response, Cellular migration/inhibition, Diazepam, Lipopolysaccharide, Macrophages/stimulation, Tnf-α, Diazepam, Tumor necrosis factor alpha, Animal cell, Animal experiment, Antiinflammatory activity, Ascites fluid cytology, Cell count, Chemotaxis, Controlled study, Drug dose comparison, Drug effect, Drug mechanism, Female, Inflammation, Inflammatory cell, Macrophage, Macrophage migration, Mononuclear cell, Mouse, Nonhuman, Polymorphonuclear cell, Single drug dose, Swiss webster mouse
Idioma
Português
Citação
Revista Brasileira de Ciências Farmaceuticas. São Paulo: Univ São Paulo, Conjunto Quimicas, v. 44, n. 4, p. 613-620, 2008.
