A method for chromosome preparations from large fish specimens using in vitro short-term treatment with colchicine
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Birkhauser Verlag Ag
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Resumo
This paper describes a new technique for preparing mitotic fish chromosomes using short-term in vitro treatment with colchicine. The results show that a large number of good quality metaphases (many suitable for chromosome banding) can be obtained by this technique, which requires an average of 1 h and 30 min for all steps. The procedure considerably reduces the time normally required for chromosome preparations in fish.
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fish cytogenetics, mitotic chromosomes, technique, colchicine, animal tissue, chromosome, cytogenetics, fish, mitosis, nonhuman, priority journal
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Inglês
Citação
Experientia, v. 49, n. 9, p. 810-813, 1993.
