Bothropoides pauloensis venom effects on isolated perfused kidney and cultured renal tubular epithelial cells
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Data
2015-09-26
Autores
Marinho, Aline Diogo
Morais, Isabel Cristina Oliveira de
Lima, Dânya Bandeira
Jorge, Antônio Rafael Coelho
Jorge, Roberta Jeane Bezerra
Menezes, Róseo Paula Pessoa Bezerra de
Mello, Clarissa Perdigão
Pereira, Gustavo José da Silva
Silveira, João Alison de Moraes
Toyama, Marcos Hikari [UNESP]
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Título de Volume
Editor
Elsevier B. V.
Resumo
Snake envenomation (Bothrops genus) is common in tropical countries and acute kidney injury is one of the complications observed in Bothrops snakebite with relevant morbidity and mortality. Here, we showed that Bothropoides pauloensis venom (BpV) decreased cell viability (IC50 of 7.5 μg/mL). Flow cytometry with annexin V and propidium iodide showed that cell death occurred predominantly by apoptosis and late apoptosis, through caspases 3 and 7 activation, mitochondrial membrane potential collapse and ROS overproduction. BpV reduced perfusion pressure, renal vascular resistance, urinary flow, glomerular filtration rate, percentage of sodium, chloride or potassium tubular transportation. These findings demonstrated that BpV cytotoxicity on renal epithelial cells might be responsible for the nephrotoxicity observed in isolated kidney.
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Palavras-chave
Bothropoides pauloensis, Cell death, Renal effects
Como citar
Toxicon : Official Journal Of The International Society On Toxinology, v. 108, p. 126-133, 2015.