Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

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Data

2016-01-01

Autores

Miyazaki, Celina Massumi
Pereira, Tamyris Paschoal
Mascagni, Daniela Branco Tavares [UNESP]
Moraes, Marli Leite de
Ferreira, Marystela

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Editor

Elsevier B. V.

Resumo

In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. The LbL film growth was monitored by surface plasmon resonance (SPR) by gold resonance angle shift analysis after each bilayer deposition. Subsequently, the films were applied as amperometric biosensors for dopamine detection using Prussian Blue (PB) as the electron mediator. The biosensor fabricated by MAO-B incorporated into liposomes composed of DPPG:POPG in the ratio (1:4) (w/w) showed the best performance with a sensitivity of 0.86 (μA cm− 2)/(mmol L− 1) and a detection limit of 0.33 mmol L− 1.

Descrição

Palavras-chave

Amperometry, Dopamine detection, Layer-by-layer, Liposomes, Monoamine oxidase-b

Como citar

Materials Science & Engineering. C, Materials For Biological Applications, v. 58, p. 310-315, 2016.

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