Publicação: A first-derivative spectrophotometric method for the determination of ciprofloxacin hydrochloride in ophthalmic solution
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Data
2012
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Scientific and Academic Publishing
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Resumo
A sensitive, economical and reproducible derivative visible-spectrophotometric method for determination of ciprofloxacin hydrochloride in ophthalmic solution was developed and validated. The method is based on the development of yellow colored product due the reaction between ciprofloxacin hydrochloride and Fe (III). After the reaction, the spectra of the solutions were recorded in the range of 360-500 nm and the first-derivative spectra were obtained by instrumental electronic differentiation using a delta lambda (Δλ) of 8 nm. Analytical responses were units of distance from the central zero base line to the negative peak obtained at 386.4 nm. The method was completely validated according to the ICH guidelines, showing accuracy, precision, selectivity, robustness and linearity. The proposed method is simple, of low cost and provides reliable results in order to be used in quality control of ophthalmic solutions containing ciprofloxacin hydrochloride as active substance.
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Inglês
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Physical Chemistry, v. 2, n. 6, p. 116-122, 2012.
