Avaliação do potencial antioxidante e antiglicante de fitoterápicos utilizados para os sintomas de climatério
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Data
2016-01-25
Autores
Gomes, Amanda da Costa [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O climatério é uma fase biológica na vida da mulher em que ocorre a transição do período reprodutivo e o não reprodutivo, caracterizado pela diminuição da produção hormonal e consequentemente a cessação permanente dos ciclos menstruais associados ao decréscimo da função ovariana. Sendo assim, a necessidade de investigação clínica em mulheres no período do climatério torna-se necessária à medida que aumenta a expectativa média de vida da população. Terapias alternativas são estudadas e avaliadas, dentre elas destacam-se as preparações à base de plantas, denominadas de fitoterápicos. Este trabalho avaliou o potencial antioxidante nos fitoterápicos a base de Cimicifuga racemosa, Trifolium pratense, Humulus lupulus e Morus nigra utilizados para sintomas do climatério, por meio dos testes in vitro de sequestro de radical livre estável DPPH, potencial redutor de ferro (FRAP), atividade sequestradora do radical óxido nítrico (NO), inibição da peroxidação lipídica (TBARS) e pelo método de inibição da hemólise oxidativa em eritrócitos humanos. Também foram avaliadas in vitro a capacidade antiglicante e antioxidante, pelos sistemas albumina sérica bovina (BSA) /ribose e do sistema BSA/Metilglioxal, na presença ou ausência do agente oxidante Cu+2, por meio do ensaio da mobilidade relativa em eletroforese. Foram quantificados os polifenóis e flavonoides totais presentes nos extratos secos. Entre os fitoterápicos, a maior atividade antioxidante tanto para o sequestro de DPPH quanto para o teste FRAP foi detectada no extrato seco de Humulus lupulus (83,56% e 46,95 µM ET/ g de extrato seco, respectivamente). Na inibição da peroxidação lipídica as menores porcentagens de TBARS foram encontradas para M. nigra (4,82%) e H. lupulus (18,26%). C. racemosa apresentou a menor formação de nitrito (34,57 µM/mL) no sequestro do radical NO. T. pratense apresentou o maior conteúdo de polifenóis (24,61 mg EAG/g de extrato seco) e flavonoides (22,79 mg ER/g de extrato seco). No ensaio de hemólise oxidativa, os extratos mostraram capacidade inibitória concentração-dependentes, enquanto para atividade antiglicante, todos os extratos demostraram potencial antiglicante.
Climacteric is a biological phase in life of woman in which occurs the transition from reproductive period and non-reproductive, characterized by decreased hormone production and consequently the permanent cessation of menstrual cycles associated with decreased ovarian function. Thus, the need for clinical investigation on women in climacteric period becomes necessary with increasing the average life expectancy of the population. Alternate therapies are being studied and assessed, among them stand out the herbal preparations, called herbal medicines. This work evaluated the antioxidant potential in Cimicifuga racemosa, Trifolium pratense, Humulus lupulus and Morus nigra, used for menopausal symptoms, through in vitro tests DPPH radical scavenging, ferric reducing antioxidant power (FRAP), scavenging of nitric oxide (NO), inhibition of lipid peroxidation (TBARS) and inhibition oxidative hemolysis human erythrocyte. Were also evaluated in vitro anti-glycation and antioxidant potential by Relative Electrophoresis Mobility (REM), using bovine serum albumin BSA with ribose and methylglyoxal, in presence or absence oxidant agent Cu2+. Total polyphenols and flavonoids present in the dry extracts were quantified. Among the herbal medicines, the highest antioxidant activity both for the DPPH radical scavenging as to the FRAP test was detected at dry extract of Humulus lupulus (83.56% and 46.95 µM ET / g of dry extract, respectively). For inhibition of lipid peroxidation the lowest percentages of TBARS were found to M. nigra (4.82%) and H. lupulus (18.26%). C. racemosa showed the lowest formation of nitrite (34.57 µM / mL) in scavenging of nitric oxide (NO). T. pratense showed the highest polyphenol content (24.61 mg GAE / g dry extract) and flavonoids (22.79 mg RE/ g of dry extract). In the oxidative haemolysis assay, extracts showed inhibitory ability concentration-dependent, while for anti-glycation activity, all extracts demonstrated inhibitory potential of glycation.
Climacteric is a biological phase in life of woman in which occurs the transition from reproductive period and non-reproductive, characterized by decreased hormone production and consequently the permanent cessation of menstrual cycles associated with decreased ovarian function. Thus, the need for clinical investigation on women in climacteric period becomes necessary with increasing the average life expectancy of the population. Alternate therapies are being studied and assessed, among them stand out the herbal preparations, called herbal medicines. This work evaluated the antioxidant potential in Cimicifuga racemosa, Trifolium pratense, Humulus lupulus and Morus nigra, used for menopausal symptoms, through in vitro tests DPPH radical scavenging, ferric reducing antioxidant power (FRAP), scavenging of nitric oxide (NO), inhibition of lipid peroxidation (TBARS) and inhibition oxidative hemolysis human erythrocyte. Were also evaluated in vitro anti-glycation and antioxidant potential by Relative Electrophoresis Mobility (REM), using bovine serum albumin BSA with ribose and methylglyoxal, in presence or absence oxidant agent Cu2+. Total polyphenols and flavonoids present in the dry extracts were quantified. Among the herbal medicines, the highest antioxidant activity both for the DPPH radical scavenging as to the FRAP test was detected at dry extract of Humulus lupulus (83.56% and 46.95 µM ET / g of dry extract, respectively). For inhibition of lipid peroxidation the lowest percentages of TBARS were found to M. nigra (4.82%) and H. lupulus (18.26%). C. racemosa showed the lowest formation of nitrite (34.57 µM / mL) in scavenging of nitric oxide (NO). T. pratense showed the highest polyphenol content (24.61 mg GAE / g dry extract) and flavonoids (22.79 mg RE/ g of dry extract). In the oxidative haemolysis assay, extracts showed inhibitory ability concentration-dependent, while for anti-glycation activity, all extracts demonstrated inhibitory potential of glycation.
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Palavras-chave
Menopausa, Climatério, Stress oxidativo, Plantas medicinais, Mulheres - envelhecimento, Menopause, Climacteric, Oxidative stress, Medicinal plants, Women - aging