Avaliação da resposta inflamatória local após aplicação de células tronco mesenquimais alogênicas em tendão flexor digital superficial de equinos
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Data
2016-02-25
Autores
Brandão, Jaqueline de Souza [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O tendão flexor digital superficial (TFDS) é uma estrutura importante para a espécie equina e para animais de alto desempenho atlético. Atualmente, a medicina regenerativa vem evoluindo de forma significativa no tratamento de diversas enfermidades, no entanto, o entendimento a respeito do comportamento celular quando do transplante de células tronco mesenquimais (CTMs) em tecido hígido ainda não é completamente conhecido. Sendo assim, este trabalho tem como objetivo a avaliação da resposta inflamatória local após aplicação de células tronco mesenquimais alogênicas derivadas do tecido adiposo (AdCTMs) em tendão equino comparado ao transplante autólogo e ao grupo controle. Para isso, 18 membros torácicos de 9 animais, divididos em três grupos, foram submetidos à aplicação de AdCTMs em tendão hígido, sendo que em Galog os animais receberam aplicação de AdCTMs alogênicas no membro torácico (MT); em Gauto, aplicação de células autólogas no MT e em Gcont aplicou-se o PBS como grupo controle. Esses animais foram avaliados por parâmetros físicos, exames ultrassonográficos e termográficos até o momento da biópsia, que foi realizada uma semana após aplicação das células autólogas e alogênicas para avaliar a possível reação inflamatória aguda, decorrente do implante de células no tecido tendíneo. O transplante alogênico de CTMs não resultou em reação adversa ou inflamatória que comprometesse o uso dessas células, elucidando a sua segurança neste trabalho. O seu comportamento mostrou-se semelhante ao implante autólogo, sendo assim, a formação de bancos de células alogênicas pode ser favorável e seguro.
The superficial digital flexor tendon (SDFT) is an important structure for the equine species and for high performance animals. Currently, regenerative medicine has evolved significantly in the treatment of several diseases, even though the cellular behavior during the implantation of mesenchymal stem cells in healthy tissue is not completely known, despite the fact that it has been tirelessly studied in the last few years. This study aims to evaluate and compare the local inflammatory response upon injection in equine tendon of either allogeneic and autologous mesenchymal stem cells derived from adipose tissue (AdMSC). For this purpose, 18 forelimbs, 9 animals were submitted to the application of AdMSC in healthy tendon, which were divided into 3 groups: in Galog the animals received application of allogeneic AdMSC in the thoracic member; in Gauto, autologous AdMSC were injected in the thoracic member; in Gcont, PBS was implanted as control group. These animals were evaluated through physical exams, ultrasound, and thermography up until the moment of the biopsy, which was performed a week after the application of AdMSC in order to evaluate the inflammatory response due to the implantation of the cells in the tendinous tissue. Biopsy samples were taken to histopathology and immunohistochemistry in order to evaluate the actions of allogeneic AdMSC. The allogeneic MSCs implantation did not result in adverse or inflammatory reaction which could compromise the use of these cells, elucidating their safety in this work. Their behavior was similar to autologous implant, and thus the formation of allogeneic cell banks could be favorable and safe.
The superficial digital flexor tendon (SDFT) is an important structure for the equine species and for high performance animals. Currently, regenerative medicine has evolved significantly in the treatment of several diseases, even though the cellular behavior during the implantation of mesenchymal stem cells in healthy tissue is not completely known, despite the fact that it has been tirelessly studied in the last few years. This study aims to evaluate and compare the local inflammatory response upon injection in equine tendon of either allogeneic and autologous mesenchymal stem cells derived from adipose tissue (AdMSC). For this purpose, 18 forelimbs, 9 animals were submitted to the application of AdMSC in healthy tendon, which were divided into 3 groups: in Galog the animals received application of allogeneic AdMSC in the thoracic member; in Gauto, autologous AdMSC were injected in the thoracic member; in Gcont, PBS was implanted as control group. These animals were evaluated through physical exams, ultrasound, and thermography up until the moment of the biopsy, which was performed a week after the application of AdMSC in order to evaluate the inflammatory response due to the implantation of the cells in the tendinous tissue. Biopsy samples were taken to histopathology and immunohistochemistry in order to evaluate the actions of allogeneic AdMSC. The allogeneic MSCs implantation did not result in adverse or inflammatory reaction which could compromise the use of these cells, elucidating their safety in this work. Their behavior was similar to autologous implant, and thus the formation of allogeneic cell banks could be favorable and safe.
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Palavras-chave
Alogênica, Reação imunológica, Células tronco mesenquimais, Inflamação, Equino, Allogeneic, Immunologic reaction, Mesenchymal stem cells, Inflammation, Equine