Participação de gambás e cães domiciliados como reservatórios de Leishmania infantum e Trypanosoma cruzi georreferenciados nos municípios da Regional de Saúde de Três Lagoas - MS
Carregando...
Data
2016-02-19
Autores
Zuque, Maria Angelina da Silva [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
A Leishmaniose Visceral e a Doença de Chagas são importantes zoonoses negligenciadas do ponto de vista de saúde pública. Animais domésticos, como o cão, e animais silvestres, como os gambás, fazem parte do ciclo destas zoonoses como fontes de infecção para os vetores. O estudo foi realizado em cinco municípios da Regional de Saúde de Três Lagoas, Mato Grosso do Sul, em 2013 e 2014, com objetivo de identificar a ocorrência da infecção natural por Leishmania spp e Trypanosoma cruzi em gambás (Didelphis albiventris) e cães domiciliados, descrever aspectos epidemiológicos dessas doenças na população canina e humana, seus vetores, e a distribuição espacial da Leishmaniose Visceral Canina usando técnicas do georrefenciamento. Amostras de sangue dos cães foram submetidas a análises sorológicas e moleculares, e a dos gambás somente as análises moleculares. Em relação à pesquisa de anticorpos contra a Leishmania spp., as provas sorológicas de 683 amostras dos cães, revelaram reagentes 320 amostras ao Dual Path Platform, 300 amostras ao Enzyme Linked Immunosorbent Assay e 116 a Reação de Imunofluorescência Indireta. Para Trypanosoma cruzi, pela Reação de Imunofluorescência Indireta, quatro amostras do município de Três Lagoas foram reagentes. Das 683 amostras de sangue total, dos cães submetidas à Reação em Cadeia pela Polimerase, todas foram negativas para Trypanosoma cruzi e obteve-se êxito na amplificação de Leishmania spp em 17 amostras. As 39 amostras de sangue total dos gambás foram negativas à Reação em Cadeia pela Polimerase para Leishmania spp. e Trypanosoma cruzi. Na área do estudo foi confirmada a infecção natural por Leishmania spp. somente nos cães domiciliados, mas não foi confirmada à infecção natural por Trypanosoma cruzi em gambás e cães domiciliados. Os casos humanos de leishmaniose persistem em algumas áreas de um município desta regional e ocorrem de forma esporádica em outros três, e um município não apresenta caso autóctone. O uso do georreferenciamento para Leishmaniose Visceral Canina, em Três Lagoas, forneceu subsídios para identificar áreas com prioridade de intervenção para o controle da doença. A presença de flebotomíneos, associado á localização geográfica, e às condições ambientais são favoráveis à sua disseminação, bem como a transmissão da doença aos cães e a humanos.
Visceral Leishmaniasis (VL ) and Chagas disease (CD ) are important neglected zoonoses in accordance with public health. Domestic animals such as dogs and wildlife such as possums , are part of these zoonoses cycles as reservoirs and sources of infection for vectors. The study was conducted in five Regional Health of the municipalities of Três Lagoas , Mato Grosso do Sul in 2013 and 2014 , in order to verify the occurrence of natural infection with Leishmania infantum and Trypanosoma cruzi in opossums (Didelphis albiventris) and the pet dogs, and describe the characteristics of diseases in human and canine population , vectors , and the spatial distribution of canine Visceral Leishmaniasis using techniques georrefenciamento. In dog´s blood there were performed serological analysis and molecular tests ande the samples of the opossums were performed molecular tests. Regarding the research for Leishmania antibodies the techniques serological to 683 dog samples, showed reagent 320 samples to Dual Path Platform, 300 samples to Enzyme Linked Immunosorbent Assay and 116 to Immunofluorescence Antibody Test. For Trypanosoma cruzi, the test Immunofluorescence Antibody Test (IFAT) four samples of Três Lagoas municipality were reactive. Of the samples from dogs 683 submitted to the Polymerase Chain Reaction were all negative by Trypanosoma cruzi gave successful amplification Leishmania spp in 17 of them. The 39 samples of the opossums were all negative by PCR to Leishmania spp. and Trypanosoma cruzi. In the study area was confirmed natural infection with Leishmania spp. only in pet dogs, but it was not confirmed to natural infection by Trypanosoma cruzi in opossums and pet dogs. Human cases of leishmaniasis persist in some areas of a municipality of this regional and occur sporadically in other three, and a municipality has no case.The use of georeferencing for Canine Visceral Leishmaniasis in TrêsLagoas allowed to identifycharacterisctis of the disease, as well areas with intervention priority to control the disease. The presence of phlebotomines, plus the favorable environmental conditions to its dissemination, allows the transmission of the disease to dogs and humans.
Visceral Leishmaniasis (VL ) and Chagas disease (CD ) are important neglected zoonoses in accordance with public health. Domestic animals such as dogs and wildlife such as possums , are part of these zoonoses cycles as reservoirs and sources of infection for vectors. The study was conducted in five Regional Health of the municipalities of Três Lagoas , Mato Grosso do Sul in 2013 and 2014 , in order to verify the occurrence of natural infection with Leishmania infantum and Trypanosoma cruzi in opossums (Didelphis albiventris) and the pet dogs, and describe the characteristics of diseases in human and canine population , vectors , and the spatial distribution of canine Visceral Leishmaniasis using techniques georrefenciamento. In dog´s blood there were performed serological analysis and molecular tests ande the samples of the opossums were performed molecular tests. Regarding the research for Leishmania antibodies the techniques serological to 683 dog samples, showed reagent 320 samples to Dual Path Platform, 300 samples to Enzyme Linked Immunosorbent Assay and 116 to Immunofluorescence Antibody Test. For Trypanosoma cruzi, the test Immunofluorescence Antibody Test (IFAT) four samples of Três Lagoas municipality were reactive. Of the samples from dogs 683 submitted to the Polymerase Chain Reaction were all negative by Trypanosoma cruzi gave successful amplification Leishmania spp in 17 of them. The 39 samples of the opossums were all negative by PCR to Leishmania spp. and Trypanosoma cruzi. In the study area was confirmed natural infection with Leishmania spp. only in pet dogs, but it was not confirmed to natural infection by Trypanosoma cruzi in opossums and pet dogs. Human cases of leishmaniasis persist in some areas of a municipality of this regional and occur sporadically in other three, and a municipality has no case.The use of georeferencing for Canine Visceral Leishmaniasis in TrêsLagoas allowed to identifycharacterisctis of the disease, as well areas with intervention priority to control the disease. The presence of phlebotomines, plus the favorable environmental conditions to its dissemination, allows the transmission of the disease to dogs and humans.
Descrição
Palavras-chave
Leishmaniose, Doença de Chagas, Reservatório, Infecção natural, Leishmaniasis, Chagas disease, Reservoir, Natural infection