Cultivo de Lentinula edodes e Pleurotus ostreatus em bagaço de cana-de-açúcar
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Data
2016-04-12
Autores
Souza, Letícia Gomes de [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O constante crescimento na produção de etanol acarreta em aumento da produção de bagaço de cana-de-açúcar, surgindo necessidade de desenvolver novas formas de aproveitamento deste produto. Sendo assim, o acréscimo de valor agregado no bagaço afim de que seja utilizado em ração animal pode ser uma alternativa economicamente interessante. Este trabalho utilizou Lentinula edodes (shiitake) e o Pleurotus ostreatus (shimeji) na biotranformação e enriquecimento do bagaço de cana-de-açúcar termo hidrolisado, considerando-se tempo e aditivos nutricionais, para obtenção de biomassa com melhores condições nutricionais e proteicas. Na primeira etapa do projeto foram testadas diversas combinações de meio a fim de se encontrar aquele mais adequado para o desenvolvimento fungico. Concluiu-se que o 1% carbonato de cálcio e 1% levedura seca são essenciais para o desenvolvimento dos microrganismos neste substrato. Com relação à quantidade de inóculo, para P. ostreatus assumiu-se 3% como ideal e para L. edodes, assumiu-se 8% como a quantidade ideal. Foram quantificados proteína bruta, lipídios, valor nutricional relativo (VNR) pelo método com Enterococcus zymogenes, toxicidade aguda realizado com Daphinia similis, DQO e DBO das amostras. Quanto a quantidade de proteína, houve um aumento significativo quando comparado ao bagaço original passando de aproximadamente 1% para 3,43% e 3,20% para L.edodes e P.ostreatus respectivamente com 45 dias de incubação as 25+/- 20C. Os valores de lipídeos foram baixos mesmo após a colonização. O valor nutricional relativo do bagaço de cana termo hidrolisado antes do tratamento microbiológico apresentava valores de 23,07% passando para 84,61% para L.edodes e para 73,07% para P.ostreatus. A toxicidade aguda apresentou uma diminuição após o desenvolvimento fúngico sendo que L.edodes apresentou-se mais eficiente na detoxificação. Os resultados de DQO e DBO também apontam para uma melhora na biodegradação do solubilizado do bagaço de cana para as duas espécies de fungos quando comparadas ao substrato original.
The constantly growing in ethanol production will lead to consequent increased production of sugarcane bagasse emerging need to develop new ways to use this product, Therefore the increase in value in order that residue to be used as forage for animal feed can be an economically interesting alternative. This paper used Lentinula edodes (shiitake) and Pleurotus ostreatus (shimeji) in biotranformação and enrichment of sugarcane bagasse term hydrolyzate, considering time and nutritional additives, to obtain biomass with better nutritional and protein conditions. In the first stage of the project were tested several combinations of substrate in order to find the most suitable for fungal development. It was concluded that 1% calcium carbonate and 1% dried yeast are essential for the development of microorganisms in the substrate. About the amount of inoculum, P. ostreatus assumed as an ideal 3% and L. edodes, 8% was assumed as the ideal amount. The following were quantified crude protein, lipids, nutritional value relative (NVR) by method with Enterococcus zymogenes, acute toxicity performed with Daphinia similis,, COD and BOD of amostras.susbstrato As the amount of protein, there was significant increase when compared to the original bagasse which has approximately 1% to 3.43% and 3.20% for L.edodes and P.ostreatus respectively 45 days incubaçõa the 25 +/- 20C . Lipid values were low even after colonization. The nutritional value of the term hydrolyzate sugarcane bagasse before the microbiological treatment had values of 23.07% and grows to 84.61 to 73.07 for L.edodes and to P.ostreat. The acute toxicity is decreasing after fungal growth and L.edodes presented more efficient in the detoxification. The results of COD and BOD also point to an improvement in the biodegradation of solubilized sugarcane bagasse for the two species of fungi when compared to the original substrate.
The constantly growing in ethanol production will lead to consequent increased production of sugarcane bagasse emerging need to develop new ways to use this product, Therefore the increase in value in order that residue to be used as forage for animal feed can be an economically interesting alternative. This paper used Lentinula edodes (shiitake) and Pleurotus ostreatus (shimeji) in biotranformação and enrichment of sugarcane bagasse term hydrolyzate, considering time and nutritional additives, to obtain biomass with better nutritional and protein conditions. In the first stage of the project were tested several combinations of substrate in order to find the most suitable for fungal development. It was concluded that 1% calcium carbonate and 1% dried yeast are essential for the development of microorganisms in the substrate. About the amount of inoculum, P. ostreatus assumed as an ideal 3% and L. edodes, 8% was assumed as the ideal amount. The following were quantified crude protein, lipids, nutritional value relative (NVR) by method with Enterococcus zymogenes, acute toxicity performed with Daphinia similis,, COD and BOD of amostras.susbstrato As the amount of protein, there was significant increase when compared to the original bagasse which has approximately 1% to 3.43% and 3.20% for L.edodes and P.ostreatus respectively 45 days incubaçõa the 25 +/- 20C . Lipid values were low even after colonization. The nutritional value of the term hydrolyzate sugarcane bagasse before the microbiological treatment had values of 23.07% and grows to 84.61 to 73.07 for L.edodes and to P.ostreat. The acute toxicity is decreasing after fungal growth and L.edodes presented more efficient in the detoxification. The results of COD and BOD also point to an improvement in the biodegradation of solubilized sugarcane bagasse for the two species of fungi when compared to the original substrate.
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Palavras-chave
Lentinula edodes, Pleurotus ostreatus., Bagaço de cana termo hidrolisado