Influência do peróxido e do uso de diferentes substâncias de combate à dor na inflamação e na expressão de neuropeptídeos pró-inflamatórios após o tratamento clareador
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Data
2016-05-13
Autores
Gallinari, Marjorie de Oliveira [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O objetivo do presente estudo foi avaliar a influência da hidrocortison e do acetaminofeno na inflamação e na expressão de neuropeptídeos por meio da análise histopatológica e imunoistoquímica. Para tanto, 63 ratos foram divididos em 3 lotes de estudo (n=21) de acordo com a terapia de combate à dor: LI- controle, LII- administração tópica de hidrocortisona por 10 minutos, depois do tratamento clareador e LIII- administração via oral de acetaminofeno 30 minutos antes do tratamento clareador com peróxido de hidrogênio a 35% e depois de 12 em 12 horas. Em todos os grupos de estudo, na maxila esquerda foi realizado o tratamento clareador placebo e a maxila direita recebeu a 3 aplicações de 15 minutos de um gel clareador a base de peróxido de hidrogênio a 35%, totalizando 45 minutos de contato do gel clareador com substrato dentário. Os momentos de eutanásia dos animais foram imediatamente após, 24 e 48 horas após o tratamento clareador. Posteriormente à eutanásia dos animais, as peças foram processadas e o primeiro molar de cada maxila foi analisado histopatologicamente quanto ao grau de inflamação e por análise de imunoistoquímica para verificarmos a presença dos neuropeptídeos SP e CGRP. Os dados obtidos foram submetidos ao teste estatístico não paramétrico Kruskal Wallis seguido do teste de Dunn para comparações individuais, sendo observado na análise histopatológica total desorganização celular, extensas áreas de necrose nos grupos clareados, e o grupo que recebeu tratamento com otosporim apresentou melhores resultados. Na análise imuno-histoquimica, obteve imunomarcação positiva em todos os grupos, inclusive controle, porém nos grupos clareados a imunomarcação foi mais forte, sendo que o grupo que recebeu tratamento com otosporim apresentou os melhores resultados. Conclui-se que o uso da hidrocortisona após tratamento clareador minimiza os efeitos colaterais deste procedimento estético.
The aim of this study was to evaluate the influence of hydrocortisone and acetaminofen substances in inflammation and neuropeptide expression by histopathologic and immunohistochemical analysis. For this, 63 rats were divided into 3 batches of study (n = 21) according to combat pain therapy: Li control LII- topical administration of Otosporin® for 10 minutes after the bleaching treatment and administration route LIIIoral Tylenol® 30 minutes before the bleaching with hydrogen peroxide at 35% and then 12 for 12 hours. In all study groups in left maxilla was performed treatment whitener placebo and right jaw received three applications of 15 minutes a whitening gel 35% hydrogen peroxide base, totaling 45 minutes of contact of the whitening gel dental substrate. The times of the animals were euthanized immediately after 24 and 48 hours after the bleaching treatment. After the euthanasia of animals, the pieces were processed and the first molar of each jaw was analyzed histologically the degree of inflammation and analysis of immunohistochemistry to verify the presence of the neuropeptides SP and CGRP. The data were submitted to statistical nonparametric Kruskal Wallis test followed by Dunn's test for individual comparisons, being observed on histopathologic total cellular disorganization analysis, extensive areas of necrosis in whitened groups, and the group that received treatment with Otosporin® showed better results. In immunohistochemical analysis, obtained positive immunostaining in all groups, including control, but the whitened immunostaining groups was stronger, and the group that received treatment with Otosporin® showed the best results. We conclude that the use of Otosporin® after bleaching treatment minimizes the side effects of this cosmetic procedure.
The aim of this study was to evaluate the influence of hydrocortisone and acetaminofen substances in inflammation and neuropeptide expression by histopathologic and immunohistochemical analysis. For this, 63 rats were divided into 3 batches of study (n = 21) according to combat pain therapy: Li control LII- topical administration of Otosporin® for 10 minutes after the bleaching treatment and administration route LIIIoral Tylenol® 30 minutes before the bleaching with hydrogen peroxide at 35% and then 12 for 12 hours. In all study groups in left maxilla was performed treatment whitener placebo and right jaw received three applications of 15 minutes a whitening gel 35% hydrogen peroxide base, totaling 45 minutes of contact of the whitening gel dental substrate. The times of the animals were euthanized immediately after 24 and 48 hours after the bleaching treatment. After the euthanasia of animals, the pieces were processed and the first molar of each jaw was analyzed histologically the degree of inflammation and analysis of immunohistochemistry to verify the presence of the neuropeptides SP and CGRP. The data were submitted to statistical nonparametric Kruskal Wallis test followed by Dunn's test for individual comparisons, being observed on histopathologic total cellular disorganization analysis, extensive areas of necrosis in whitened groups, and the group that received treatment with Otosporin® showed better results. In immunohistochemical analysis, obtained positive immunostaining in all groups, including control, but the whitened immunostaining groups was stronger, and the group that received treatment with Otosporin® showed the best results. We conclude that the use of Otosporin® after bleaching treatment minimizes the side effects of this cosmetic procedure.
Descrição
Palavras-chave
Clareamento dental, Inflamação, Hidrocortisona, Acetaminofeno, Substância P, CGRP, Dental bleaching, Pulpit, Hydrocortisone, CGRP, Acetaminophen