Proteinograma e concentração de imunoglobulina G séricos em potros, do nascimento aos trinta dias de vida, tratados com plasma
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Data
2016-07-04
Autores
Cunha Neto, Campo Amor Vieira da [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Este trabalho teve por objetivo avaliar o proteinograma e concentrações séricas de IgG em potros do nascimento aos trinta dias, antes e depois de mamarem colostro e serem tratados com plasma, bem como avaliar a qualidade do colostro e plasma utilizados. Para tal, foram utilizados 20 potros e suas respectivas mães, além de quatro animais doadores (plasma). Foram colhidas amostras de sangue dos potros em cinco momentos (M1= zero, M2= dez, M3= 24, M4= 48 horas e M5= 30 dias de vida), sangue e colostro das éguas progenitoras no momento do parto. Amostras de soro plasma foram avaliadas a concentrações de Proteína total e albumina por técnica colorimétrica e refratometria. O proteinograma sérico foi obtido através de eletroforese em gel de agarose e a globulina total foi estimada através de cálculo matemático. Foi avaliado a densidade do colostro e a concentração de IgG total de todas as amostras foi determinada por imunodifusão radial e um novo sistema de ELISA. A média ± desvio padrão da concentração sérica de IgG dos potros de M1 a M5, avaliada por ELISA, foi de 15±8 mg/dL, 2.408±608 mg/dL, 2.282±783 mg/dL, 2.364±784 mg/dL e 1.414±586 mg/dL, respectivamente. Os níveis séricos e colostrais de IgG (ELISA) foram de 1746±505 mg/dL e 7714±2619 mg/dL, respectivamente. Houve forte correlação entre os resultados obtidos por ambos os testes de quantificação de IgG. Todavia, a concentração de IgG determinada por IDRS, em todas as amostras, foram estatisticamente maiores que as determinadas por ELISA.
The aim of this study was to evaluate the protein concentrations and serum IgG in foals birth to thirty days, before and after suckling colostrum and be treated with plasma and evaluate the quality of colostrum and plasma used. Twenty foals and their mothers were used and four donor animals (plasma). colts blood samples were collected in five times (M1 = zero, M2 = ten M3 = 24, M4 = 48 hours and M5 = 30 days), blood and colostrum progenitor mares at foaling. Plasma Serum samples were evaluated for total protein and albumin concentrations and refraction by colorimetric technique. Serum protein was obtained by electrophoresis on an agarose gel, and the total globulin has been estimated through mathematical calculation. The mean ± standard deviation of the serum IgG concentration of foals group of the M1 at M5, measured by ELISA, was of 15±8 mg/dL, 2,408±608 mg/dL, 2,282±783 mg/dL, 2,364±784 mg/dl and 1.414±586 mg/dL, respectively. Serum IgG and colostral (ELISA) were 1.746±505 mg/dL and 7.714±2619 mg/dl, respectively. There was a strong correlation between the results obtained by both quantitation of IgG tests. However, the IgG concentration determined by SRID in all samples were statistically higher than those obtained by ELISA.
The aim of this study was to evaluate the protein concentrations and serum IgG in foals birth to thirty days, before and after suckling colostrum and be treated with plasma and evaluate the quality of colostrum and plasma used. Twenty foals and their mothers were used and four donor animals (plasma). colts blood samples were collected in five times (M1 = zero, M2 = ten M3 = 24, M4 = 48 hours and M5 = 30 days), blood and colostrum progenitor mares at foaling. Plasma Serum samples were evaluated for total protein and albumin concentrations and refraction by colorimetric technique. Serum protein was obtained by electrophoresis on an agarose gel, and the total globulin has been estimated through mathematical calculation. The mean ± standard deviation of the serum IgG concentration of foals group of the M1 at M5, measured by ELISA, was of 15±8 mg/dL, 2,408±608 mg/dL, 2,282±783 mg/dL, 2,364±784 mg/dl and 1.414±586 mg/dL, respectively. Serum IgG and colostral (ELISA) were 1.746±505 mg/dL and 7.714±2619 mg/dl, respectively. There was a strong correlation between the results obtained by both quantitation of IgG tests. However, the IgG concentration determined by SRID in all samples were statistically higher than those obtained by ELISA.
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Palavras-chave
Potros, Imunoglobulinas G, Eletroforese, Ensaio imunoenzimático, Plasma, Foals, Electrophoresis, Enzyme-Linked Immunosorbent assay