Efeito da radiação ultravioleta associada à Riboflavina em epimastigotas de Trypanosoma cruzi: estudo In vitro
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Data
2016-06-30
Autores
López-Castañeda, Brayan [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
A Doença de Chagas é uma zoonose causada pelo Trypanosoma cruzi (T. cruzi), um protozoário flagelado que se transmite por múltiplas vias e que se estabelece no sangue e diversos órgãos dos indivíduos infectados. A doença é endêmica no Brasil e representa uma das causas frequentes de morte, pois as alternativas terapêuticas além de serem limitadas, apresentam resultados razoavelmente efetivos. O presente estudo teve como objetivo avaliar in vitro os efeitos da Terapia Fotodinâmica utilizando dois protocolos de radiação de luz ultravioleta associados à riboflavina, sobre epimastigotas de T. cruzi. Resultados dos testes de viabilidade celular MTT e Azul de Tripan, e do acompanhamento da cinética de crescimento, não registraram efeitos citotóxicos imediatos ou tardios sobre os protozoários. Contrario ao esperado, os resultados indicam um aumento na multiplicação das epimastigotas tratadas. Com este estudo permitiu-se concluir que, a radiação de luz ultravioleta associada à riboflavina, não é eficaz no controle e/ou eliminação de epimastigotas do T. cruzi, e que novos estudos deveram ser desenvolvidos para tentar identificar as variáveis que influenciam a multiplicação do protozoário.
Chagas disease is a zoonotic disease caused by Trypanosoma cruzi (T. cruzi) a flagellate protozoan that is transmitted by multiple pathways and that is established in blood and various organs of infected individuals. The disease is endemic in Brazil and is one of the common causes of death, for therapeutic alternatives as well as being limited, present fairly effective results. This study aimed to evaluate in vitro the effects of photodynamic therapy using two ultraviolet radiation protocols associated with riboflavin on Trypanosoma cruzi epimastigote. Results of cell viability tests MTT and Trypan Blue, and monitoring the growth kinetics, reported no immediate cytotoxic effects or late on protozoa. Interestingly, the results suggest an increase in proliferation of treated epimastigote. This study allowed to conclude that the radiation of ultraviolet light associated with riboflavin, is not effective in controlling and / or eliminating Epimastigote T. cruzi, and that further studies were to be developed to help identify the variables that influence the multiplication the protozoan.
Chagas disease is a zoonotic disease caused by Trypanosoma cruzi (T. cruzi) a flagellate protozoan that is transmitted by multiple pathways and that is established in blood and various organs of infected individuals. The disease is endemic in Brazil and is one of the common causes of death, for therapeutic alternatives as well as being limited, present fairly effective results. This study aimed to evaluate in vitro the effects of photodynamic therapy using two ultraviolet radiation protocols associated with riboflavin on Trypanosoma cruzi epimastigote. Results of cell viability tests MTT and Trypan Blue, and monitoring the growth kinetics, reported no immediate cytotoxic effects or late on protozoa. Interestingly, the results suggest an increase in proliferation of treated epimastigote. This study allowed to conclude that the radiation of ultraviolet light associated with riboflavin, is not effective in controlling and / or eliminating Epimastigote T. cruzi, and that further studies were to be developed to help identify the variables that influence the multiplication the protozoan.
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Palavras-chave
Doença de Chagas, Chagas disease, Trypanosoma cruzi, Terapia fotodinâmica, Photodynamic therapy, Epimastigotas, Riboflavin, Riboflavina