Osteopontin in Seminal Plasma and Sperm Membrane of Dogs
Nenhuma Miniatura disponível
Data
2009-07-01
Autores
Souza, Fabiana Ferreira de [UNESP]
Chirinea, V. H. [UNESP]
Martins, M. I. M.
Lopes, Maria Denise [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Wiley-Blackwell Publishing, Inc
Resumo
The objective of this work was to describe the presence of osteopontin (OPN) in canine seminal plasma and sperm membranes. A pool of seminal plasma and sperm membrane extract from 30 dogs was used. Polyacrylamide electrophoresis gels were performed and the bands were transferred to nitrocellulose paper and Western blot was undertaken using an antibody anti-OPN. Two and 12 bands were marked in the seminal plasma (77.2 and 15.6 kDa) and sperm membrane extracts (70.6-26.6 kDa), respectively. However, from 12 marked bands in the sperm membrane extract, only three (46.4, 37.7 and 36.5 kDa) were strongly marked. We conclude that, seminal plasma and sperm membranes from dogs contain different isoforms of OPN; yet, further studies will be necessary to determine their function in this species.
Descrição
Palavras-chave
Como citar
Reproduction In Domestic Animals. Malden: Wiley-blackwell Publishing, Inc, v. 44, p. 283-286, 2009.