Publicação: Parthenogenetic development of domestic cat oocytes treated with ionomycin, cycloheximide, roscovitine and strontium
Carregando...
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Tipo
Artigo
Direito de acesso
Acesso restrito
Resumo
The objective was to evaluate the parthenogenetic activation of domestic cat oocytes. Cumulus-oocyte complexes matured for 36 h were subjected to three protocols of parthenogenetic activation: Group 1 - ionomycin + cycloheximide; Group 2 - ionomycin + roscovitine; and Group 3 - ionomycin + strontium. As a control, a fourth group of oocytes were cultured in the absence of any activation agent. In all groups, embryos were cultured in SOFaa for 72 h after activation and evaluated for activation rate, cleavage, and embryonic development using Hoechst 33342. There were no significant differences among the three treated groups for rates of activated oocytes (70.1 +/- 4.3, 75.5 +/- 4.7, and 61.9 +/- 7.2%, for Treatments 1, 2, and 3 respectively; mean +/- SEM), or cleavage (48.1 +/- 5.9, 47.4 +/- 3.8, and 33.3 +/- 6.8%). However, activation and cleavage rates were higher (P < 0.05) than those in the control group (35.5 +/- 6.4 and 11.8 +/- 4.0%). There were no significant differences among treatment groups for proportion of embryos with 2-10 cells, 10-16 cells, and morulas. In the Control group, the embryo production rate was lower (P < 0.05), although the activation rate was high. The authors concluded that all three treatments effectively induced parthenogenetic activation of domestic cat oocytes. However, to optimize the use of strontium and roscovitine, a dose response and the effect of the presence of Ca(++) in the medium requires further study. (C) 2010 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
Strontium, Roscovitine, Cycloheximide, Artificial activation, Feline oocytes
Idioma
Inglês
Como citar
Theriogenology. New York: Elsevier B.V., v. 74, n. 4, p. 596-601, 2010.
