Taenia saginata: Differential diagnosis of human taeniasis by polymerase chain reaction-restriction fragment length polymorphism assay
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Data
2005-08-01
Autores
Nunes, Caris Maroni [UNESP]
Dias, AKK
Dias, FEF
Aoki, S. M.
de Paula, H. B.
Lima, LGF
Garcia, José Fernando [UNESP]
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Editor
Elsevier B.V.
Resumo
Speciation of Taenia in human stool is important because of their different clinical and epidemiological features. DNA analysis has recently become possible which overcomes the problems of differentiating human taeniid cestodes morphologically. In the present study, we evaluated PCR coupled to restriction fragment length polymorphism to differentiate Taenia solium from Taenia saginata eggs present in fecal samples from naturally infected patients. A different Dral-RFLP pattern: a two-band pattern (421 and 100 bp) for T saginata and a three-band pattern (234, 188, and 99 bp) for T solium was observed allowing the two species to be separated.. The lower detection limit of the PCR-RFLP using a non-infected fecal sample prepared with a given number of T saginata eggs was 34 eggs in 2 g stool sediment. The 521 bp mtDNA fragment was detected in 8 out of 12 Taenia sp. carriers (66.6%). of these, three showed a T solium pattern and five a T saginata pattern. (c) 2005 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
taeniasis, PCR-RFLP, diagnosis, fecal samples, Taemia saginata, Taenia solium
Como citar
Experimental Parasitology. San Diego: Academic Press Inc. Elsevier B.V., v. 110, n. 4, p. 412-415, 2005.