Estudo clínico e laboratorial da pneumonia de bezerros induzida pela inoculação intrabronquial de Mannheimia haemolytica
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Data
2003-02-01
Autores
Fagliari, J.J. [UNESP]
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Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária
Resumo
Foram utilizados 14 bezerros da raça Holandesa entre 15 e 30 dias de idade, sete inoculados com 5ml de solução salina fosfato-tamponada Dulbecco (DPBS) e sete inoculados com 5x10(9) UFC de Mannheimia haemolytica/5 ml de DPBS, via intrabronquial. O exame clínico e as colheitas de sangue e de lavado broncoalveolar foram realizados antes e 4, 6, 12 e 24 horas após a inoculação. O exame histopatológico dos pulmões revelou lesões de pneumonia fibrinosa hemorrágica. Os sinais clínicos surgiram quatro horas após a inoculação das bactérias. Os sete bezerros enfermos apresentavam apatia, respiração abdominal, alteração dos ruídos respiratórios, hipertermia, elevação das freqüências cardíaca e respiratória e arritmia cardíaca. Os exames de laboratório mostraram aumento na contagem de hemácias e do volume globular, indicando hemoconcentração. A diminuição de PO2 e a elevação de PCO2 sugerem inadequada ventilação pulmonar. Observaram-se neutrofilia e alto número de neutrófilos e monócitos no fluido broncoalveolar. O pH do sangue e as concentrações plasmáticas de HCO3, Na e K não apresentaram alterações significativas.
Seven control Holstein calves were inoculated intrabronchially with 5.0ml of Dulbecco s phosphate-buffered saline solution (DPBS). Other seven calves were inoculated with 5´ 10(9) Mannheimia haemolytica organisms/5.0 ml of DPBS. Bronchoalveolar fluid and blood samples were collected immediately before and at 4, 6, 12, and 24 hours after inoculation. Clinical examination was performed at the same time points. Histologically haemorragic and fibrinotic pneumonia was observed. Seven sick calves had lethargy, abdominal respiratory pathern, altered respiratory sounds, hypertemia, increased heart and respiratory rates, and cardiac arrhythmia. Pneumonic calves showed higher packed cell volume and red cell count, suggesting hemoconcentration. Pneumonic calves also had decreased PO2 and increased PCO2 suggesting poor pulmonary ventilation. Neutrophilia and high neutrophil and monocyte counts in bronchoalveolar fluid were detected due to pulmonar inflammation. Blood pH and plasma concentrations of HCO3, Na, and K had no significant changes.
Seven control Holstein calves were inoculated intrabronchially with 5.0ml of Dulbecco s phosphate-buffered saline solution (DPBS). Other seven calves were inoculated with 5´ 10(9) Mannheimia haemolytica organisms/5.0 ml of DPBS. Bronchoalveolar fluid and blood samples were collected immediately before and at 4, 6, 12, and 24 hours after inoculation. Clinical examination was performed at the same time points. Histologically haemorragic and fibrinotic pneumonia was observed. Seven sick calves had lethargy, abdominal respiratory pathern, altered respiratory sounds, hypertemia, increased heart and respiratory rates, and cardiac arrhythmia. Pneumonic calves showed higher packed cell volume and red cell count, suggesting hemoconcentration. Pneumonic calves also had decreased PO2 and increased PCO2 suggesting poor pulmonary ventilation. Neutrophilia and high neutrophil and monocyte counts in bronchoalveolar fluid were detected due to pulmonar inflammation. Blood pH and plasma concentrations of HCO3, Na, and K had no significant changes.
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Palavras-chave
Calf, pneumonia, Mannheimia haemolytica, Diagnosis, Bezerro, Pneumonia, Mannheimia haemolytica, Diagnóstico
Como citar
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 55, n. 1, p. 8-14, 2003.