Isolation and characterization of a satellite DNA family in Achirus lineatus (Teleostei : Pleuronectiformes : Achiridae)
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Data
2005-11-01
Autores
de Azevedo, MFC
Oliveira, C.
Martins, C.
Wasko, A. P.
Foresti, F.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Springer
Resumo
Agarose gels stained with Ethidium bromide and Southern blot experiments of HindIII-digested genomic DNA of Achirus lineatus evidenced the presence of monomers and multimers of a DNA segment of about 200 bp, named here Al-HindIII sequence. No signals were observed in Southern blot experiments with genomic DNA of other flatfish species. The DNA sequencing of four recombinant clones showed that Al-HindIII sequences had 204 bp and were 63.72% AT-rich. FISH experiments using a Al-HindIII sequence as probe showed bright signals in the centromeric position of all chromosomes of A. lineatus.
Descrição
Palavras-chave
chromosome structure, fluorescent in situ hybridization (FISH), Pleuronectiformes, satellite DNA
Como citar
Genetica. Dordrecht: Springer, v. 125, n. 2-3, p. 205-210, 2005.