Publicação: Isolation and characterization of a satellite DNA family in Achirus lineatus (Teleostei : Pleuronectiformes : Achiridae)
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Springer
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Agarose gels stained with Ethidium bromide and Southern blot experiments of HindIII-digested genomic DNA of Achirus lineatus evidenced the presence of monomers and multimers of a DNA segment of about 200 bp, named here Al-HindIII sequence. No signals were observed in Southern blot experiments with genomic DNA of other flatfish species. The DNA sequencing of four recombinant clones showed that Al-HindIII sequences had 204 bp and were 63.72% AT-rich. FISH experiments using a Al-HindIII sequence as probe showed bright signals in the centromeric position of all chromosomes of A. lineatus.
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chromosome structure, fluorescent in situ hybridization (FISH), Pleuronectiformes, satellite DNA
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Inglês
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Genetica. Dordrecht: Springer, v. 125, n. 2-3, p. 205-210, 2005.
