Short Report: Heterogeneity of Leishmania infantum chagasi Kinetoplast DNA in Teresina (Brazil)
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2010-05-01
Autores
Alonso, Diego Peres
Costa, Dorcas Lamounier
de Mendonca, Ivete Lopes
Nery Costa, Carlos Henrique
Martins Ribolla, Paulo Eduardo
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Amer Soc Trop Med & Hygiene
Resumo
Leishmania infantum chagasi is the causative agent of visceral leishmaniasis in Brazil. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of kinetoplast DNA (kDNA) minicircles was used to evaluate genetic profiles of 48 Leishmania infantum chagasi strains from dog and human parasite cultures, fresh collected dog bone marrow aspirates, and from infected sand flies. Results revealed that heterogeneity in kDNA minicircles depends mostly on the source of the samples, with cultured parasites showing a high degree of homogeneity.
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American Journal of Tropical Medicine and Hygiene. Mclean: Amer Soc Trop Med & Hygiene, v. 82, n. 5, p. 819-821, 2010.