Fracionamento de cobre em proteínas do plasma, músculo e fígado de tilápia do Nilo
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Data
2012-01-01
Autores
Neves, Renato C. F. [UNESP]
Lima, Paula M. [UNESP]
Baldassini, Welder A. [UNESP]
Santos, Felipe A. [UNESP]
Moraes, Paula M. [UNESP]
Castro, Gustavo R. [UNESP]
Padilha, Pedro M. [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Química
Resumo
Copper fractionation in plasma, muscle and liver of Nile tilapia was performed after protein separation by 2D-PAGE. SR XRF analysis indicated the presence of copper in three protein spots of plasma, and in two protein spots of muscle and liver, respectively. Copper ions were found to be distributed mostly in proteins that had a molar mass of less than 54 kDa and greater than 13 kDa and a pI in the 5.3-9.3 range. The copper concentration bound to these proteins was determined by GFAAS which showed concentrations in the 1.20-4.82 mg g-1 range.
Descrição
Palavras-chave
Nile tilapia, metalloproteins, metallomics
Como citar
Química Nova. Sociedade Brasileira de Química, v. 35, n. 3, p. 493-498, 2012.