Characterization and localization of dynein and myosins V and VI in the ovaries of queen bees

Nenhuma Miniatura disponível

Data

2010-10-01

Autores

Patricio, Karina [UNESP]
Calabria, Luciana Karen
Peixoto, Pablo Marco
Espindola, Foued Salmen
Da Cruz-Landim, Carminda [UNESP]

Título da Revista

ISSN da Revista

Título de Volume

Editor

Portland Press

Resumo

The presence of myosin and dynein in the ovaries of both Apis mellifera and Scaptotrigona postica was investigated in extracts and in histological sections. In the ovary extracts, motor proteins, myosins V, VI and dynein were detected by Western blot. In histological sections, they were detected by immunocytochemistry, using a mouse monoclonal antibody against the intermediary chain of dynein and a rabbit polyclonal antibody against the myosin V head domain. The myosin VI tail domain was recognized by a pig polyclonal antibody. The results show that these molecular motors are expressed in the ovaries of both bee species with few differences in location and intensity, in regions where movement of substances is expected during oogenesis. The fact that antibodies against vertebrate proteins recognize proteins of bee species indicates that the specific epitopes are evolutionarily well preserved.

Descrição

Palavras-chave

cytoskeleton, dynein, myosin V, myosin VI, oogenesis

Como citar

Cell Biology International. London: Portland Press Ltd, v. 34, n. 10, p. 1041-1047, 2010.