Lactic acid production by new Lactobacillus plantarum LMISM6 grown in molasses: optimization of medium composition
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Data
2011-03-01
Autores
Coelho, L. F. [UNESP]
Lima, C. J. B. de [UNESP]
Rodovalho, C. M. [UNESP]
Bernardo, M. P. [UNESP]
Contiero, Jonas [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Brazilian Society of Chemical Engineering
Resumo
A Plackett-Burman experimental design was used to evaluate seven medium components added to molasses (corn steep liquor, sodium acetate, magnesium sulfate, manganese sulfate, ammonium citrate, potassium phosphate and Tween 80). Corn steep liquor (CSL), K2HPO4 and Tween 80 increased lactic acid production. The concentrations of these three components as well as the molasses were further optimized using the response surface method. A maximal lactic acid production of 94.8 g L-1 was obtained when the concentrations of molasses, CSL, K2HPO4 and Tween 80 were 193.50 g L-1, 37.50 mL L-1, 2.65 g L-1 and 0.83 mL L-1, respectively. However, in both shaker and bioreactor, approximately one fourth of the sugar added initially was not utilized after 48 hours of fermentation. Future studies that consider high conversion of sugar into final product as well as high volumetric productivity are necessary to improve the fermentation process and to reduce the downstream costs.
Descrição
Palavras-chave
Plackett-Burman, Corn steep liquor, Response surface methodology, Medium optimization, Potassium phosphate, Tween
Como citar
Brazilian Journal of Chemical Engineering. Brazilian Society of Chemical Engineering, v. 28, n. 1, p. 27-36, 2011.