Production of xylanase and CMCase on solid state fermentation in different residues by Thermoascus aurantiacus miehe
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Data
2005-09-01
Autores
Silva, Roberto da [UNESP]
Lago, Ellen S. [UNESP]
Merheb, Carolina W. [UNESP]
Macchione, Mariana M. [UNESP]
Park, Yong Kun [UNESP]
Gomes, Eleni [UNESP]
Título da Revista
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Título de Volume
Editor
Sociedade Brasileira de Microbiologia
Resumo
O emprego de residuos como matéria prima é importante como estrategia governamental e para o balanço ambiental. O propósito deste trabalho foi estudar a produção de CMCase e xilanase de uma linhagem de Thermoascus aurantiacus isolado de solo brasileiro em fermentação em estado sólido (SSF) usando diferentes resíduos agrícolas (farelo de trigo, bagaço de cana, bagaço de laranja, sabugo de milho, grama verde, grama seca, serragem de eucalipto e palha de milho) como substratos sem enriquecimento dos meios e caracterizar as enzimas. O estudo das enzimas hemiceluloliticas extracelulares mostrou que o fungo T. arantiacus é mais xilanolítico do que celulolítico. Ele produziu maiores níveis das enzimas em meios contendo sabugo de milho, grama e palha de milho. Todas as enzimas foram estáveis por 24 h à temperatura ambiente numa ampla faixa de pH (3,0 - 9,0) e também foram estáveis a 60ºC por 1 h. O pH ótimo e temperatura ótima para xilanase e CMCase foram 5,0- 5,5 e 5,0 e 75ºC, respectivamente. O microrganismo cresceu muito bem estacionariamente no meio simples, de baixo custo. As enzimas estáveis secretadas extracelularmente apresentam as características necessárias para sua aplicação industrial.
The use of waste as raw material is important for government economy and natural balance. The purpose of this work was to study the production of CMCase and xylanase by a Brazilian strain of Thermoascus aurantiacus in solid state fermentation (SSF) using different agricultural residues (wheat bran, sugarcane bagasse, orange bagasse, corncob, green grass, dried grass, sawdust and corn straw) as substrates without enrichment of the medium and characterize the crude enzymes.The study of the extracellular cellulolytic and hemicellulolytic enzymes showed that T. arantiacus is more xylanolytic than cellulolytic. The highest levels of enzymes were produced in corncob, grasses and corn straw. All the enzymes were stable at room temperature by 24 h over a broad pH range (3.0-9.0) and also were stable at 60ºC for 1 h. The optimum pH and temperature for xylanase and CMCase were 5.0-5.5 and 5.0 and 75ºC, respectively. The microorganism grew quickly in stationary, simple and low cost medium. The secreted extracellular enzymes presented properties that match with those frequently required in industrial environment.
The use of waste as raw material is important for government economy and natural balance. The purpose of this work was to study the production of CMCase and xylanase by a Brazilian strain of Thermoascus aurantiacus in solid state fermentation (SSF) using different agricultural residues (wheat bran, sugarcane bagasse, orange bagasse, corncob, green grass, dried grass, sawdust and corn straw) as substrates without enrichment of the medium and characterize the crude enzymes.The study of the extracellular cellulolytic and hemicellulolytic enzymes showed that T. arantiacus is more xylanolytic than cellulolytic. The highest levels of enzymes were produced in corncob, grasses and corn straw. All the enzymes were stable at room temperature by 24 h over a broad pH range (3.0-9.0) and also were stable at 60ºC for 1 h. The optimum pH and temperature for xylanase and CMCase were 5.0-5.5 and 5.0 and 75ºC, respectively. The microorganism grew quickly in stationary, simple and low cost medium. The secreted extracellular enzymes presented properties that match with those frequently required in industrial environment.
Descrição
Palavras-chave
Thermoascus aurantiacus, Xylanase, CMCase, Solid state fermentation (SSF), agricultural residues, Thermoascus aurantiacus, Xilanase, CMCase, Fermentação em estado sólido, Resíduos agrícolas
Como citar
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 36, n. 3, p. 235-241, 2005.