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  • ItemArtigo
    The degradation of chicken feathers by Ochrobactrum intermedium results in antioxidant and metal chelating hydrolysates and proteolytic enzymes for staphylococcal biofilm dispersion
    (2023-06-01) de Menezes, Cíntia Lionela Ambrosio [UNESP]; Boscolo, Maurício [UNESP]; da Silva, Roberto [UNESP]; Gomes, Eleni [UNESP]; da Silva, Ronivaldo Rodrigues [UNESP]; Universidade Estadual Paulista (UNESP)
    The increase in the generation of chicken feathers, due to the large production of the poultry industry, has created the need to search for ecologically safer ways to manage these residues. As a sustainable alternative for recycling keratin waste, we investigated the ability of the bacterium Ochrobactrum intermedium to hydrolyze chicken feathers and the valorization of the resulting enzymes and protein hydrolysate. In submerged fermentation with three different inoculum sizes (2.5, 5.0, and 10.0 mg of bacterial cells per 50 mL of medium), the fastest degradation of feathers was achieved with 5.0 mg cells, in which a complete decomposition of the substrate (96 h) and earlier peaks of keratinolytic and caseinolytic activities were detected. In the resulting protein hydrolysate, we noticed antioxidant and Fe2+ and Cu2+ chelating activities. ABTS scavenging, Fe3+-reducing ability and metal chelating activities of the fermentative samples followed the same trend of feather degradation; as feather mass decreased in the media, these activities increased. Furthermore, we noticed about 47% and 60% dispersion of established 7-day biofilms formed by S. aureus after enzymatic treatment for 5 h and 24 h, respectively. These findings highlight the potential use of this bacterium as an environmentally friendly alternative to treat this poultry waste and offer valuable products.
  • ItemArtigo
    Functional characteristics of an α-L-arabinofuranosidase secreted by the basidiomycete Coriolopsis byrsina
    (2023-01-01) de Oliveira Nascimento, Carlos Eduardo [UNESP]; de Menezes, Cíntia Lionela Ambrosio [UNESP]; Boscolo, Maurício [UNESP]; da Silva, Roberto [UNESP]; Gomes, Eleni [UNESP]; da Silva, Ronivaldo Rodrigues [UNESP]; Universidade Estadual Paulista (UNESP)
    α-L-arabinofuranosidases can be produced by fungi, bacteria, and plants and are responsible for the cleavage of α-1,2, α-1,3, and α-1,5 glycosidic bonds in arabinose residues. Few recent studies have focused on the production of these enzymes by species of basidiomycetes. In the present study, we purified an α-L-arabinofuranosidase from the fungus Coriolopsis byrsina and evaluated its functional biochemical properties. The purified enzyme had an estimated molecular mass of 55 kDa with maximum activity at pH 3.5–4.5 and 50 °C, stability in the range of pH 3–8 at 4 °C for 24 h, and from 10 to 60 °C for 1 h. The enzyme was also stable in 5–10% ethanol after 24 h at 28 °C, retaining more than 60% activity. A negative effect on catalysis was noted for all evaluated ions, especially Fe3+ and Hg2+, retaining 25% and 33% activity, respectively, at 10 mmol L−1, and L-arabinose, with 50% activity at 0.2 mol L−1 and 30% at 0.9 mol L−1. The enzyme was active on p-nitrophenyl-α-L-arabinofuranoside (pNPA), p-nitrophenyl-β-D-xylopyranoside, linear 1,5 α-L-arabinan and xylan from beechwood. Kinetic assays under pNPA substrate indicated a KM of 3.45 ± 0.9 mmol L−1 and Vmax of 198.2 ± 24 μmol min−1 mg−1. These findings can serve as a reference for further tests of this enzyme aimed at improving the aroma of beverages and formulating enzyme cocktails for plant biomass degradation.
  • ItemResenha
    β-glucosidase: An overview on immobilization and some aspects of structure, function, applications and cost
    (2023-07-01) Mól, Paula Chequer Gouveia [UNESP]; Júnior, José Carlos Quilles; Veríssimo, Lizzy Ayra Alcântara; Boscolo, Mauricio [UNESP]; Gomes, Eleni [UNESP]; Minim, Luis Antonio; Da Silva, Roberto [UNESP]; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP); Federal University of Lavras; Federal University of Vicosa
    β-glucosidases constitute a heterogeneous group of well-characterized and biologically important hydrolytic enzymes which act on different substrates. They have been widely utilized in biotechnology. Nowadays, immobilized β-glucosidase has become one of the most important biocatalytic systems used for many industrial purposes, especially in the biofuel, food, pharmaceutical and beverage industries. Immobilized β-glucosidase has the advantages of usually being chemically and thermally more stable, easily separated from the product, possible to reuse and can replace chemical catalysts in some industrial applications. From both a scientific and application point of view, interest in developing new support materials and new protocols for β-glucosidase immobilization has grown. This review focused on originality and novelty on how an immobilization system for β-glucosidases should be and what is missing for really efficient applications. For that, the main methods used for the immobilization of β-glucosidase and the description of the different support materials, with emphasis on their advantages and disadvantages; the main physical-chemical characteristics of immobilized enzymes; the applications of the pure or mixed immobilized enzyme; and a discussion on the β-glucosidase market and cost are presented. Finally, the overall conclusion is given along with a mention of perspectives and future directions.
  • ItemArtigo
    Xylose consumption and ethanol production by Pichia guilliermondii and Candida oleophila in the presence of furans, phenolic compounds, and organic acids commonly produced during the pre-treatment of plant biomass
    (2023-06-01) da Silva, Ronivaldo Rodrigues [UNESP]; Zaiter, Mohammed Anas [UNESP]; Boscolo, Maurício [UNESP]; da Silva, Roberto [UNESP]; Gomes, Eleni [UNESP]; Universidade Estadual Paulista (UNESP)
    For 2G ethanol production, pentose fermentation and yeast tolerance to lignocellulosic hydrolyzate components are essential to improve biorefinery yields. Generally, physicochemical pre-treatment methodologies are used to facilitate access to cellulose and hemicellulose in plant material, which consequently can generate microbial growth inhibitory compounds, such as furans, weak acids, and phenolic compounds. Because of the unsatisfactory yield of wild-type Saccharomyces cerevisiae during pentose fermentation, the search for xylose-fermenting yeasts tolerant to microbial growth inhibitors has gained attention. In this study, we investigated the ability of the yeasts Pichia guilliermondii G1.2 and Candida oleophila G10.1 to produce ethanol from xylose and tolerate the inhibitors furfural, 5-hydroxymethylfurfural (HMF), acetic acid, formic acid, ferulic acid, and vanillin. We demonstrated that both yeasts were able to grow and consume xylose in the presence of all single inhibitors, with greater growth limitation in media containing furfural, acetic acid, and vanillin. In saline medium containing a mixture of these inhibitors (2.5–3.5 mM furfural and HMF, 1 mM ferulic acid, 1–1.5 mM vanillin, 10–13 mM acetic acid, and 5–7 mM formic acid), both yeasts were able to produce ethanol from xylose, similar to that detected in the control medium (without inhibitors). In future studies, the proteins involved in the transport of pentose and tolerance to these inhibitors need to be investigated.
  • ItemArtigo
    Modified and Optimized Glass Electrode for pH Measurements in Hydrated Ethanol Fuel
    (2022-11-01) Atilio, Natalia Cambiaghi; Fertonani, Fernando Luis [UNESP]; Oliveira, Elcio Cruz de; Pontifical Catholic University of Rio de Janeiro; Universidade Estadual Paulista (UNESP); PETROBRAS S.A
    One of the quality control parameters of ethanol fuel is pH, established by the Brazilian standard ABNT NBR 10891, whose scope is specific for hydrated ethanol fuel, and by the American standard ASTM D 6423, which focuses on anhydrous ethanol fuel. This study presented a modified and optimized structure using a single solvent, both for the glass electrode and the external reference electrode, to minimize the presence of the liquid junction potential for measuring the pH of hydrated ethanol fuel. The Box–Behnken design enabled us to determine the optimal condition expected for the new measurement system, which was compared with the systems proposed by the standard references and the turning range of acid–base indicators using parametric and nonparametric tests. The results revealed that the pH values obtained by the different systems are statistically different, and that only the values obtained by this proposal are suitable for the pH range found by the indicators. The optimized electrode presented an adequate response sensitivity to the Nernst equation, having an operational behavior adequate for the modified and optimized glass electrode for pH measurements in hydrated ethanol fuel.
  • ItemArtigo
    The First Report of In Vitro Antifungal and Antibiofilm Photodynamic Activity of Tetra-Cationic Porphyrins Containing Pt(II) Complexes against Candida albicans for Onychomycosis Treatment
    (2023-05-01) da Rosa Pinheiro, Ticiane; Dantas, Gabrielle Aguiar; da Silva, Jean Lucas Gutknecht; Leal, Daniela Bitencourt Rosa; da Silva, Ricardo Barreto; de Lima Burgo, Thiago Augusto; Santos, Roberto Christ Vianna; Iglesias, Bernardo Almeida [UNESP]; Federal University of Santa Maria; Universidade Estadual Paulista (UNESP)
    Onychomycosis is a prevalent nail fungal infection, and Candida albicans is one of the most common microorganisms associated with it. One alternative therapy to the conventional treatment of onychomycosis is antimicrobial photoinactivation. This study aimed to evaluate for the first time the in vitro activity of cationic porphyrins with platinum(II) complexes 4PtTPyP and 3PtTPyP against C. albicans. The minimum inhibitory concentration of porphyrins and reactive oxygen species was evaluated by broth microdilution. The yeast eradication time was evaluated using a time-kill assay, and a checkerboard assay assessed the synergism in combination with commercial treatments. In vitro biofilm formation and destruction were observed using the crystal violet technique. The morphology of the samples was evaluated by atomic force microscopy, and the MTT technique was used to evaluate the cytotoxicity of the studied porphyrins in keratinocyte and fibroblast cell lines. The porphyrin 3PtTPyP showed excellent in vitro antifungal activity against the tested C. albicans strains. After white-light irradiation, 3PtTPyP eradicated fungal growth in 30 and 60 min. The possible mechanism of action was mixed by ROS generation, and the combined treatment with commercial drugs was indifferent. The 3PtTPyP significantly reduced the preformed biofilm in vitro. Lastly, the atomic force microscopy showed cellular damage in the tested samples, and 3PtTPyP did not show cytotoxicity against the tested cell lines. We conclude that 3PtTPyP is an excellent photosensitizer with promising in vitro results against C. albicans strains.
  • ItemArtigo
    Water Reactivity in Electrified Interfaces: The Simultaneous Production of Electricity, Hydrogen, and Hydrogen Peroxide at Room Temperature
    (2023-04-25) Santos, Leandra P.; Lermen, Diana; Yoshimura, Rafael Galiza; da Silva, Bruno Leuzinger; Galembeck, André; Burgo, Thiago A. L. [UNESP]; Galembeck, Fernando; Galembetech Consultores e Tecnologia Ltda.; Universidade Estadual de Campinas (UNICAMP); Universidade Federal de Pernambuco (UFPE); Universidade Estadual Paulista (UNESP)
    Hygroelectric cells deliver hydrogen, hydrogen peroxide, and electric current simultaneously at room temperature from liquid water or vapor. Different cell arrangements allowed the electrical measurements and the detection and measurement of the reaction products by two methods each. Thermodynamic analysis shows that water dehydrogenation is a non-spontaneous reaction under standard conditions, but it can occur within an open, non-electroneutral system, thus supporting the experimental results. That is a new example of chemical reactivity modification in charged interfaces, analogous to the hydrogen peroxide formation in charged aqueous aerosol droplets. Extension of the experimental methods and the thermodynamic analysis used in this work may allow the prediction of interesting new chemical reactions that are otherwise unexpected. On the other hand, this adds a new facet to the complex behavior of interfaces. Hygroelectric cells shown in this work are built from commodity materials, using standard laboratory or industrial processes that are easily scaled up. Thus, hygroelectricity may eventually become a source of energy and valuable chemicals.
  • ItemArtigo
    Oxidation of hydrochar produced from byproducts of the sugarcane industry for the production of humic-like substances: Characterization and interaction study with Cu(II)
    (2023-05-01) Moura, Bernardo R. [UNESP]; Santos, Vinicius S. [UNESP]; Metzker, Gustavo [UNESP]; Ferreira, Odair P.; Bisinoti, Márcia C. [UNESP]; Boscolo, Mauricio [UNESP]; Moreira, Altair B. [UNESP]; Universidade Estadual Paulista (UNESP); Federal University of Ceará; Universidade Estadual de Londrina (UEL)
    Humic-like substances (HLS) are molecules extracted in an alkaline medium from different materials that have not been subjected to the natural process of humification that occurs in the soil. HLS have the potential to be used as organic fertilizers due to their ability to incorporate micronutrients such as Cu(II) and Co(II); in addition, they represent an alternative for the remediation of contaminated areas due to their high affinity for metals. HLS can be extracted from hydrochar (HC) but only with low yields of approximately 5%. Therefore, the present study aimed to increase the amount of HLS extracted from the HC produced from byproducts of the sugarcane industry through the oxidation of HC with HNO3. HLS extracted from oxidized and unoxidized HC were characterized by CHNS analysis and 13C CPMAS NMR. The interaction between HLS and Cu(II) was studied by molecular fluorescence quenching (EEM-PARAFAC) and applying the Ryan and Weber complexation model. The oxidation of HC with HNO3 allowed high yields of extracted HLS of above 80%. The oxidation carried out with 30% HNO3 for 2 h showed the best result, since the HLS30%(2h) were extracted with a very high yield (88.3%) in a short period of time. Oxidation promoted a decrease in HLS aromaticity and an increase in oxygen and nitrogen groups. HLS showed high affinity for Cu(II), as evidenced by the high logK values (between 5.5 and 5.9). HLS extracted from oxidized HCs showed higher complexation capacity due to the greater incorporation of the oxygenated groups promoted by oxidation, which are fundamental during the interaction with metallic cations. Therefore, the oxidation of HC substantially increased the production of HLS, representing a big advance for the production of carbonaceous materials with higher added value from byproducts of the sugarcane industry produced on a large scale in Brazil.
  • ItemArtigo
    Antimicrobial activity of water-soluble tetra-cationic porphyrins on Pseudomonas aeruginosa
    (2023-06-01) Urquhart, Carolina Gonzalez; Pinheiro, Ticiane da Rosa; da Silva, Jean Lucas Gutknecht; Leal, Daniela Bitencourt Rosa; Burgo, Thiago Augusto Lima [UNESP]; Iglesias, Bernardo Almeida; Santos, Roberto Christ Vianna; Universidade Federal de Santa Maria; Universidade Federal de Sergipe (UFS); Universidade Estadual Paulista (UNESP)
    This manuscript presents the cytotoxicity, antimicrobial activity, antibiofilm preliminary properties, and associated therapy with commercial drugs using water-soluble tetra-cationic porphyrins against Pseudomonas aeruginosa. Two commercial tetra-cationic porphyrins were tested against a standard strain of P. aeruginosa 01 (PA01) in antibacterial activity assays under dark conditions and irradiated with white light for 120 min. Porphyrin 4-H2TMePor showed better antimicrobial activity and was chosen for further tests. Increased minimum inhibitory concentration was observed in the presence of reactive oxygen species, suggesting that photooxidation was mediated by the singlet oxygen production. In the time-kill curve assay, 4-H2TMePor inhibited bacterial growth in 90 min of irradiation. The checkerboard assay revealed synergistic interactions. Biofilms of the standard PA01 strain and three clinical isolates were formed. The biofilm destruction assay was more efficient for PA01, significantly reducing the biofilm biomass formed compared to the positive control. The associated treatment to destroy the biofilm potentiated a significant decrease in the biofilm biomass compared to the positive control. The photosensitizer did not damage human keratinocytes or mouse fibroblasts in the cytotoxicity assays, demonstrating the safety of using 4-H2TMePor. Atomic force microscopy indicated lower adhesion force, higher cell wall deformation, and higher dissipation energy in the treated control compared to untreated PA01. Given our findings, it is evident that water-soluble tetra-cationic porphyrins have excellent antimicrobial and a preliminary antibiofilm activity against Gram-negative bacteria, proving to be a potential photosensitizer for clinical use.
  • ItemArtigo
    Low-cost triboelectric nanogenerator based on aseptic carton package
    (2023-03-01) Moreira, Kelly S.; Santos da Campo, Yan A.; Lorenzett, Ezequiel; Burgo, Thiago A.L. [UNESP]; Federal University of Santa Maria; Universidade Estadual Paulista (UNESP)
    Triboelectric nanogenerators (TENGs) are efficient systems that convert mechanical energy to electrical energy. Based on contact electrification, an emerging powerful tool in energy harvesting technologies, most TENGs are constructed using expensive materials (e.g. gold and platinum) and costly processes. Aseptic carton packages (ACP) are plastic-laminated materials basically composed of paperboard, polyethylene and aluminum, but having all parts required for a TENG device: dielectric inductor and metal. Using the concept implicit in the triboelectric series where hydrophilic materials acquire a positive net charge while hydrophobic ones develop negative net charge upon contact, ACP was used as the main component of a sandwich-type triboelectric nanogenerator (ACP-TENG). Electrostatic potential mapping confirmed that pristine polyethylene is a negative charge inductor while after its oxidation develops an excess positive charge. Potentials as high as 200 V are reached with ACP-TENG during mechanical loads, charging a 10 μF capacitor to 3.5 V in less than 10 min. Continuous open-circuit voltage of roughly 80 V is developed under pressure-relaxation cycles at 1.8 Hz, reaching an output peak current of ∼400 nA. The efficiency of ACP-TENG is comparable to nanoengineered devices, but costing only a fraction of them. We believe that the ACP-TENG reported here is a reliable way to construct low-cost energy harvesting devices and it can be used as an environmentally friendly destination/solution for food packaging.
  • ItemArtigo
    Thermal and electrochemical studies of the interaction of organophilic montmorillonite and chlorpyrifos
    (2023-01-01) Lupi, Caroline Polini [UNESP]; Ferreira, Antonio Ap. Pupim [UNESP]; Guizellini, Filipe Corrêa [UNESP]; Pastre, Iêda Aparecida [UNESP]; Yamanaka, Hideko [UNESP]; Fertonani, Fernando Luis [UNESP]; Universidade Estadual Paulista (UNESP); SPR Soluções Metrológicas LTDA; Itean College of Botucatu
    This work investigates the thermal and electrochemical interaction of montmorillonite Clay (Mt) and a modified montmorillonite Clay (Mt-M) with a peptide sequence, which mimics the active site of the enzyme acetylcholinesterase, as well as their interactions with the chlorpyrifos pesticide. The methods used include thermal analysis techniques (DSC), X-ray diffraction, scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The electrochemical study was carried out in KCl 0.1 mol L−1, pH 7, containing a redox species Fe(CN)6–3/−4. The electrode surface coverage (θ) was 0.42 for Mt and 0.65 for Mt-M. The interpretation of the electrochemical impedance spectroscopy results was based on the charge transfer reaction involving the [Fe(CN)6]−3/−4 species at high frequencies, followed by diffusion through the monolayer at low frequencies. The heterogeneity of the Mt and Mt-M film formed on electrode surface limits the transport of [Fe(CN)6]−3/−4 through the montmorillonite layer since the Mt particles formed non-regular overlapping plates, observed under microscopy. The DSC analyses revealed disorganization in the structure of the organophilic clay with the insertion of the pesticide, reflected in the appropriate waters of the system.
  • ItemArtigo
    In vitro porphyrin-based photodynamic therapy against mono and polyculture of multidrug-resistant bacteria isolated from integumentary infections in animals
    (2022-12-01) Machado, Carolina S.; Seeger, Marlane G.; Moreira, Kelly S.; Burgo, Thiago A.L. [UNESP]; Iglesias, Bernardo A.; Vogel, Fernanda S.F.; Cargnelutti, Juliana F.; Universidade Federal de Sergipe (UFS); Universidade Estadual Paulista (UNESP)
    Multidrug-resistant (MDR) organisms have been frequently isolated from integumentary lesions of animals, and these lesions are usually infected by more than one pathogen. This study evaluated an in vitro antimicrobial photodynamic therapy (aPDT) using two water-soluble tetra-cationic porphyrins (3-H2TMeP and 4-H2TMeP) against mono and polyculture of MDR bacteria isolated from dogs, cats, and horses. Ten isolates of MDR bacteria (two of each species: Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Serratia marcescens, and Staphylococcus pseudointermedius) were used to evaluate aPDT against the monoculture using a non-cytotoxic concentration of 3-H2TMeP and 4-H2TMeP porphyrins (40 µM), with 30 min of light irradiation in Gram-positive and 90 min for Gram-negative bacteria. The aPDT using the 4-H2TMeP porphyrin was also tested against five different polycultures (Coagulase positive Staphylococcus (CPS) and Pseudomonas sp.; E. coli and Proteus sp.; Pseudomonas sp. and Proteus sp.; CPS and E. coli; and CPS and Proteus sp.) for 90 min. The efficacy of both treatments was evaluated by plating the solution exposed to light or kept in the dark and counting the colonies forming units after 24 h of incubation at 37 °C. Atomic force microscope analysis was used to map bacteria morphological changes and extract adhesion force parameters from the bacteria membranes. Only the 4-H2TMeP porphyrin had antibacterial activity against MDR bacteria in monoculture, especially S. pseudointermedius and P. aeruginosa. In polyculture, the 4-H2TMeP porphyrin reduced bacterial concentrations (p < 0.05) in the associations of E. coli and S. pseudointermedius, P. aeruginosa and S. pseudointermedius, and P. aeruginosa and P. mirabilis. These results showed that aPDT using 4-H2TMeP is a good option for future associations of aPDT and other therapies or in vivo research.
  • ItemArtigo
    AFM advanced modes for dental and biomedical applications
    (2022-12-01) Burgo, ThiagoA.L. [UNESP]; Pereira, Gabriel Kalil Rocha; Iglesias, Bernardo Almeida; Moreira, Kelly S.; Valandro, Luiz Felipe; Universidade Estadual Paulista (UNESP); Universidade Federal de Sergipe (UFS)
    Several analytical methods have been employed to elucidate bonding mechanisms between dental hard tissues, luting agents and restorative materials. Atomic Force Microscopy (AFM) imaging that has been extensively used in materials science, but its full capabilities are poorly explored by dental research community. In fact, commonly used to obtain topographic images of different surfaces, it turns out that AFM is an underestimated technique considering that there are dozens of basic and advanced modes that are scarcely used to explain properties of biomaterials. Thus, this paper addresses the use of phase-contrast imaging, force-distance curves, nanomechanical and Kelvin probe force techniques during AFM analysis to explore topological, nanomechanical and electrical properties of Y-TZP samples modified by different surface treatments, which has been widely used to promote adhesive enhancements to such substrate. The AFM methods are capable of access erstwhile inaccessible properties of Y-TZP which allowed us to describe its adhesive properties correctly. Thus, AFM technique emerges as a key tool to investigate the complex nature of biomaterials and highlighting its inherent interdisciplinarity that can be successfully used for bridging fragmented disciplines such as solid-state physics, microbiology and dental sciences.
  • ItemArtigo
    Fungal Growth on Solid Substrates: a Physiological Overview
    (Elsevier B.V., 2018-01-01) Gomes, Eleni [UNESP]; Silva, Roberto da [UNESP]; Pereira, Josiani de Cassia [UNESP]; Ladino-Orjuela, Guillermo [UNESP]; Pandey, A.; Larroche, C.; Soccol, C. R.; Universidade Estadual Paulista (UNESP)
  • ItemEditorial
    Introduction-Pollution and Fish Health in Tropical Ecosystems a Brief Summary on Current Challenges and Perspectives
    (Crc Press-taylor & Francis Group, 2014-01-01) Almeida, Eduardo Alves de [UNESP]; Ferreira Rodrigues, Aline Cristina [UNESP]; Oliveira Ribeiro, Ciro Alberto de; DeAlmeida, E. A.; Ribeiro, CAD; Universidade Estadual Paulista (UNESP); Univ Fed Parana
  • ItemArtigo
    EVALUATION OF DNA ADDUCTS FORMED BY LIPID PEROXIDATION BY-PRODUCTS
    (Blackwell Science Publ, 2012-01-01) Machado Garcia, Camila Carriao; Friedmann Angeli, Jose Pedro; Almeida, Eduardo Alves de [UNESP]; Gennari de Medeiros, Marisa Helena; Di Mascio, Paolo; Abele, D.; VazquezMedina, J. P.; ZentenoSavin, T.; Universidade de São Paulo (USP); Universidade Estadual Paulista (UNESP)
  • ItemArtigo
    EVALUATION OF OXIDATIVE DNA DAMAGE IN AQUATIC ANIMALS: COMET ASSAYS AND 8-OXO-7,8-DIHIDRO-2 '-DEOXYGUANOSINE LEVELS
    (Blackwell Science Publ, 2012-01-01) Friedmann Angeli, Jose Pedro; Martinez, Glaucia Regina; Motta, Flavia Daniela; Almeida, Eduardo Alves de [UNESP]; Gennari de Medeiros, Marisa Helena; Di Mascio, Paolo; Abele, D.; VazquezMedina, J. P.; ZentenoSavin, T.; Universidade de São Paulo (USP); Univ Fed Parana; Universidade Estadual Paulista (UNESP)
  • ItemArtigo
    EVALUATION OF MALONDIALDEHYDE LEVELS
    (Blackwell Science Publ, 2012-01-01) Miyamoto, Sayuri; Almeida, Eduardo Alves de [UNESP]; Nogueira, Lilian [UNESP]; Gennari de Medeiros, Marisa Helena; Di Mascio, Paolo; Abele, D.; VazquezMedina, J. P.; ZentenoSavin, T.; Universidade de São Paulo (USP); Universidade Estadual Paulista (UNESP)
  • ItemArtigo
    ROLE OF SINGLET MOLECULAR OXYGEN IN THE OXIDATIVE DAMAGE TO BIOMOLECULES
    (Blackwell Science Publ, 2012-01-01) Ronsein, Graziella Eliza; Martinez, Glaucia Regina; Almeida, Eduardo Alves de [UNESP]; Miyamoto, Sayuri; Medeiros, Marisa Helena Gennari de; Di Mascio, Paolo; Abele, D.; VazquezMedina, J. P.; ZentenoSavin, T.; Universidade de São Paulo (USP); Univ Fed Parana; Universidade Estadual Paulista (UNESP)
  • ItemArtigo
    EVALUATION OF GLUTATHIONE STATUS IN AQUATIC ORGANISMS
    (Blackwell Science Publ, 2012-01-01) Almeida, Eduardo Alves de [UNESP]; Humberto Silva, Danilo Grunig [UNESP]; Dias Bainy, Afonso Celso; Freitas, Florencio Porto; Motta, Flavia Daniela; Gomes, Osmar Francisco; Gennari de Medeiros, Marisa Helena; Di Mascio, Paolo; Abele, D.; VazquezMedina, J. P.; ZentenoSavin, T.; Universidade Estadual Paulista (UNESP); Universidade Federal de Santa Catarina (UFSC); Universidade de São Paulo (USP)