The use of standard methodology for determination of antifungal activity of natural products against medical yeasts Candida sp and Cryptococcus sp
Carregando...
Data
2007-09-01
Autores
Scorzoni, Liliana [UNESP]
Benaducci, Tatiane [UNESP]
Almeida, Ana Marisa Fusco [UNESP]
Silva, Dulce Helena Siqueira [UNESP]
Bolzani, Vanderlan da Silva [UNESP]
Mendes Giannini, Maria José Soares [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Microbiologia
Resumo
Metodologias para determinar atividade antifúngica foram desenvolvidas com adaptações para avaliar produtos naturais. As mais usadas são bioautografia e o método de difusão em agar, que empregam meios de cultura complexos, não definidos. Neste estudo são discutidos os métodos para determinação de atividade antifúngica de produtos naturais e é recomendado o uso do micrométodo modificado segundo o documento M27A2 do CLSI.
Methodology for testing natural compounds for determination of antifungal activity had been developed with adaptations. The most used are bioautography and agar diffusion with a complex and no defined media. In this study, different methods for determination of antifungal activity of natural products are discussed and the use of M27-A2 microdilution test from CLSI (Clinical and Laboratory Standards Institute, 2002), as general standard methodology for testing plant extracts activity is recommended.
Methodology for testing natural compounds for determination of antifungal activity had been developed with adaptations. The most used are bioautography and agar diffusion with a complex and no defined media. In this study, different methods for determination of antifungal activity of natural products are discussed and the use of M27-A2 microdilution test from CLSI (Clinical and Laboratory Standards Institute, 2002), as general standard methodology for testing plant extracts activity is recommended.
Descrição
Palavras-chave
produtos naturais, atividade antifúngica, Candida sp, Cryptococcus sp, natural products, antifungal activity, Candida sp, Cryptococcus sp
Como citar
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 38, n. 3, p. 391-397, 2007.