Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
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Brazilian Archives of Biology and Technology
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Acesso aberto
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Resumo
Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.
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determination, selective inactivation, Phaseolus vulgaris
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Inglês
Citação
Brazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 41, n. 1, p. 17-25, 1998.
