Seminal analysis, cryogenic preservation, and fertility in matrinxã fish, Brycon cephalus (Günther, 1869)
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Data
2006-07-01
Autores
Ninhaus-Silveira, Alexandre [UNESP]
Foresti, Fausto [UNESP]
Veríssimo-Silveira, Rosicleire [UNESP]
Senhorini, José Augusto
Título da Revista
ISSN da Revista
Título de Volume
Editor
Brazilian Archives of Biology and Technology
Resumo
Visando o desenvolvimento das técnicas de reprodução em peixes, foi realizada a caracterização e a criopreservação do sêmen de Brycon cephalus. As características seminais observadas foram: sêmen de coloração leitosa quase transparente, com um volume médio de 4mL e concentração espermática de 9.617±1.630 x 10(6) espermatozóides/mm³. Os espermatozóides (comprimento = 31,288±4,47 µm) são do tipo aquasperm, apresentando uma pequena cabeça arredondada (comprimento= 1,752±0,18 µm; largura = 1,752±0,17 µm) sem vesícula acrosomal, com um núcleo com cromatina altamente compactada, formando grumos grosseiros e um complexo centriolar localizado na fossa nuclear; uma peça intermediária (comprimento = 2,561±0,44 µm) que se estreita no sentido antero-posterior, um canal citoplasmático e um flagelo (comprimento = 29,521±4,37 µm). Os testes de fertilização com sêmen descongelado demonstraram um efeito significante (alfa = 0,05) no aumento da fertilidade quando utilizado o diluente tipo B, para ambos os tipos de palhetas, 0,5mL e 4,0mL. Não foi constatado efeito significativo (alfa = 0,05) entre a utilização de palhetas de 0,5mL ou de 4,0mL em relação à porcentagem de eclosão.
Aiming to improve fish reproduction techniques, the characterization and cryopreservation of semen of Brycon cephalus were performed. The seminal characteristics observed were: an almost transparent, milky semen with a mean volume of 4mL, and sperm concentration of 9.617±1.630 x 10(6) spermatozoa/mm³. Spermatozoa (length = 31.288 ±4.47µm) were of the aquasperm type and displayed a small, round head (length = 1.727 ±0.18 µm; width = 1.752 ±0.17µm) without acrosomal vesicle, nucleus with highly condensed chromatin forming coarse clots and centriolar complex located in the nuclear fossa; a midpiece (length = 2.561±0.44µm), narrowed rearward, with a cytoplasmic canal; and a flagellum (length = 29.521 ±4.37µm). Fertilization tests with thawed semen demonstrated a significant effect (alpha= 0.05) on the increase of thawed semen fertility with diluent type B in both 0.5mL and 4.0 mL straws. No significant effect (alpha= 0.05) on hatching rate was observed in both straw sizes used.
Aiming to improve fish reproduction techniques, the characterization and cryopreservation of semen of Brycon cephalus were performed. The seminal characteristics observed were: an almost transparent, milky semen with a mean volume of 4mL, and sperm concentration of 9.617±1.630 x 10(6) spermatozoa/mm³. Spermatozoa (length = 31.288 ±4.47µm) were of the aquasperm type and displayed a small, round head (length = 1.727 ±0.18 µm; width = 1.752 ±0.17µm) without acrosomal vesicle, nucleus with highly condensed chromatin forming coarse clots and centriolar complex located in the nuclear fossa; a midpiece (length = 2.561±0.44µm), narrowed rearward, with a cytoplasmic canal; and a flagellum (length = 29.521 ±4.37µm). Fertilization tests with thawed semen demonstrated a significant effect (alpha= 0.05) on the increase of thawed semen fertility with diluent type B in both 0.5mL and 4.0 mL straws. No significant effect (alpha= 0.05) on hatching rate was observed in both straw sizes used.
Descrição
Palavras-chave
Semen characteristics, Cryopreservation, Brycon cephalus, Ultrastructure, Fish
Como citar
Brazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 49, n. 4, p. 651-659, 2006.