Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis
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Data
2011-10-01
Autores
Oliveira, Antonio Martins [UNESP]
Oliva Neto, Pedro de [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Brazilian Archives of Biology and Technology
Resumo
The optimization of autolysis of Saccharomyces cerevisiae from brewery was studied aiming at the maximum ribonucleic acid extraction and yeast extract production. The best conditions for yeast autolysis was 55.2ºC, pH= 5.1 and 9.8% NaCl for 24h of processing, without the NH3 use. In these conditions, the RNA yield was 89.7%, resulting in 51.3% of dehydrated yeast extract with 57.9% protein. The use of 12.2% NH3 at 60ºC after autolysis (8h) and plasmolysis (8h) was not viable due to the reduction in the RNA yield from 89.7to78.4%. on the other hand, the thermal shock at 60ºC for 15 minutes prior to autolysis provided an increase in the yield from 89.7 to 91.4%. The autolysis, including NaCl plasmolysis in the optimized conditions was efficient, economic and with short time, thus usable for industrial purpose to obtain more valuable products such as yeast extract enriched in RNA and/or protein, for different applications.
Descrição
Palavras-chave
Autolysis, NaCl plasmolysis, yeast extract, RNA extraction, nucleotides
Como citar
Brazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 54, n. 5, p. 1007-1018, 2011.